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Journal Articles Rice Science Year : 2022

Diversity of Sodium Transporter HKT1;5 in Genus Oryza

Shalini Pulipati
  • Function : Author
Suji Somasundaram
  • Function : Author
Nitika Rana
  • Function : Author
Kavitha Kumaresan
  • Function : Author
Mohamed Shafi
  • Function : Author
Gothandapani Sellamuthu
  • Function : Author
Deepa Jaganathan
  • Function : Author
Prasanna Venkatesan Ramaravi
  • Function : Author
S. Punitha
  • Function : Author
Kalaimani Raju
  • Function : Author
Shrikant Mantri
  • Function : Author
R. Sowdhamini
  • Function : Author
Ajay Parida
  • Function : Author
Gayatri Venkataraman
  • Function : Author


Asian cultivated rice shows allelic variation in sodium transporter, OsHKT1;5, correlating with shoot sodium exclusion (salinity tolerance). These changes map to intra/extracellularly-oriented loops that occur between four transmembrane-P loop-transmembrane (MPM) motifs in OsHKT1;5. HKT1;5 sequences from more recently evolved Oryza species (O. sativa/O. officinalis complex species) contain two expansions that involve two intracellularly oriented loops/helical regions between MPM domains, potentially governing transport characteristics, while more ancestral HKT1;5 sequences have shorter intracellular loops. We compared homology models for homoeologous OcHKT1;5-K and OcHKT1;5-L from halophytic O. coarctata to identify complementary amino acid residues in OcHKT1;5-L that potentially enhance affinity for Na+. Using haplotyping, we showed that Asian cultivated rice accessions only have a fraction of HKT1;5 diversity available in progenitor wild rice species (O. nivara and O. rufipogon). Progenitor HKT1;5 haplotypes can thus be used as novel potential donors for enhancing cultivated rice salinity tolerance. Within Asian rice accessions, 10 non-synonymous HKT1;5 haplotypic groups occur. More HKT1;5 haplotypic diversities occur in cultivated indica gene pool compared to japonica. Predominant Haplotypes 2 and 10 occur in mutually exclusive japonica and indica groups, corresponding to haplotypes in O. sativa salt-sensitive and salt-tolerant landraces, respectively. This distinct haplotype partitioning may have originated in separate ancestral gene pools of indica and japonica, or from different haplotypes selected during domestication. Predominance of specific HKT1;5 haplotypes within the 3 000 rice dataset may relate to eco-physiological fitness in specific geo-climatic and/or edaphic contexts.
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hal-04023359 , version 1 (10-03-2023)


Attribution - NonCommercial - NoDerivatives



Shalini Pulipati, Suji Somasundaram, Nitika Rana, Kavitha Kumaresan, Mohamed Shafi, et al.. Diversity of Sodium Transporter HKT1;5 in Genus Oryza. Rice Science, 2022, 29 (1), pp.31-46. ⟨10.1016/j.rsci.2021.12.003⟩. ⟨hal-04023359⟩
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