Skip to Main content Skip to Navigation
Journal articles

The cell biologist's guide to super-resolution microscopy

Abstract : Fluorescence microscopy has become a ubiquitous method to observe the location of specific molecular components within cells. However, the resolution of light microscopy is limited by the laws of diffraction to a few hundred nanometers, blurring most cellular details. Over the last two decades, several techniques-grouped under the 'super-resolution microscopy' moniker-have been designed to bypass this limitation, revealing the cellular organization down to the nanoscale. The number and variety of these techniques have steadily increased, to the point that it has become difficult for cell biologists and seasoned microscopists alike to identify the specific technique best suited to their needs. Available techniques include image processing strategies that generate super-resolved images, optical imaging schemes that overcome the diffraction limit and sample manipulations that expand the size of the biological sample. In this Cell Science at a Glance article and the accompanying poster, we provide key pointers to help users navigate through the various super-resolution methods by briefly summarizing the principles behind each technique, highlighting both critical strengths and weaknesses, as well as providing example images.
Complete list of metadatas

Cited literature [121 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-02869863
Contributor : Christophe Leterrier <>
Submitted on : Tuesday, June 16, 2020 - 12:27:40 PM
Last modification on : Wednesday, June 17, 2020 - 3:27:09 AM

Files

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2020-12-11

Please log in to resquest access to the document

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Collections

Citation

Guillaume Jacquemet, Alexandre Carisey, Hellyeh Hamidi, Ricardo Henriques, Christophe Leterrier. The cell biologist's guide to super-resolution microscopy. Journal of Cell Science, Company of Biologists, 2020, ⟨10.1242/jcs.240713⟩. ⟨hal-02869863⟩

Share

Metrics

Record views

61