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Journal Articles Virulence Year : 2024

The Salmonella virulence protein PagN contributes to the advent of a hyper-replicating cytosolic bacterial population

Emilie Barilleau
  • Function : Author
  • PersonId : 1151104
Jérôme Trotereau
Michael Koczerka
Yves Le Vern
Julien Pichon
Philippe Velge

Abstract

Salmonella enterica subspecies enterica serovar Typhimurium is an intracellular pathogen that invades and colonizes the intestinal epithelium. Following bacterial invasion, Salmonella is enclosed within a membrane-bound vacuole known as a Salmonella-containing vacuole (SCV). However, a subset of Salmonella has the capability to prematurely rupture the SCV and escape, resulting in Salmonella hyper-replication within the cytosol of epithelial cells. A recently published RNA-seq study provides an overview of cytosolic and vacuolar upregulated genes and highlights pagN vacuolar upregulation. Here, using transcription kinetics, protein production profile, and immunofluorescence microscopy, we showed that PagN is exclusively produced by Salmonella in SCV. Gentamicin protection and chloroquine resistance assays were performed to demonstrate that deletion of pagN affects Salmonella replication by affecting the cytosolic bacterial population. This study presents the first example of a Salmonella virulence factor expressed within the endocytic compartment, which has a significant impact on the dynamics of Salmonella cytosolic hyper-replication.
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hal-04600884 , version 1 (04-06-2024)

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Sébastien Holbert, Emilie Barilleau, Jin Yan, Jérôme Trotereau, Michael Koczerka, et al.. The Salmonella virulence protein PagN contributes to the advent of a hyper-replicating cytosolic bacterial population. Virulence, 2024, 15 (1), pp.2357670. ⟨10.1080/21505594.2024.2357670⟩. ⟨hal-04600884⟩
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