Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Non‐targeted 13 C metabolite analysis demonstrates broad re‐orchestration of leaf metabolism when gas exchange conditions vary

Abstract : It is common practice to manipulate CO2 and O2 mole fraction during gas-exchange experiments to suppress or exacerbate photorespiration, or simply carry out CO2 response curves. In doing so, it is implicitly assumed that metabolic pathways other than carboxylation and oxygenation are altered minimally. In the past few years, targeted metabolic analyses have shown that this assumption is incorrect, with changes in the tricarboxylic acid cycle, anaplerosis (phosphoenolpyruvate carboxylation), and nitrogen or sulphur assimilation. However, this problem has never been tackled systematically using non-targeted analyses to embrace all possible affected metabolic pathways. Here, we exploited combined NMR, GC–MS, and LC–MS data and conducted non-targeted analyses on sunflower leaves sampled at different O2/CO2 ratios in a gas exchange system. The statistical analysis of nearly 4,500 metabolic features not only confirms previous findings on anaplerosis or S assimilation, but also reveals significant changes in branched chain amino acids, phenylpropanoid metabolism, or adenosine turn-over. Noteworthy, all of these pathways involve CO2 assimilation or liberation and thus affect net CO2 exchange. We conclude that manipulating CO2 and O2 mole fraction has a broad effect on metabolism, and this must be taken into account to better understand variations in carboxylation (anaplerotic fixa- tion) or apparent day respiration.
Liste complète des métadonnées

https://hal.inrae.fr/hal-03033342
Contributeur : Olivier Dupre <>
Soumis le : mardi 1 décembre 2020 - 12:39:05
Dernière modification le : samedi 12 décembre 2020 - 03:12:49

Fichier

 Accès restreint
Fichier visible le : 2021-12-01

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

Citation

Cyril Abadie, Julie Lalande, Anis Limami, Guillaume Tcherkez. Non‐targeted 13 C metabolite analysis demonstrates broad re‐orchestration of leaf metabolism when gas exchange conditions vary. Plant, Cell and Environment, Wiley, 2020, pp.1-13. ⟨10.1111/pce.13940⟩. ⟨hal-03033342⟩

Partager

Métriques

Consultations de la notice

20