As a part of the biogeochemical cycle, nutrient translocation plays an important role in enhancing the capacity of perennial plants to grow in nutrient-poor soils. Although leaf translocation has been extensively studied, nutrient translocation between wood rings has received considerably less attention, primarily because of methodological constraints. This study aimed to (i) evaluate the effects of different drying techniques on Ca, K, and Mn concentrations, (ii) calibrate a semiquantitative method for obtaining ring-to-ring nutrient concentrations along wood cores, and (iii) develop a complete calculation chain for nutrient translocation. Three pairs of cores per tree were extracted from nine oaks, and three drying methods-103 • C, 65 • C, and freeze-drying-were applied to each core pair. For each core pair, the first core was analyzed using ITRAX. The second core was analyzed using ICP-OES following the mineralization of a 20 mg wood sample. Ca, K, and Mn concentrations and wood density were not affected by the drying methods (p > 0.05 for Ca, K, and Mn). After upscaling at the stand level, the total translocation was 10.8 ± 5.5 kg ha -1 , 14.8 ± 11.4 kg ha -1 , and 2.6 ± 0.9 kg ha -1 for Ca, K, and Mn, respectively, after 45 growing years. The total Ca, K, and Mn translocation showed a strong tree effect, partly explained by tree diameter. The study findings suggest that similar measurements can be performed on all wood cores sampled in previous studies and stored after air-drying. These results provide a reference for future analyses of Ca, K, and Mn translocations in different species from wide geographic areas.