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Article Dans Une Revue Nanoscale Année : 2021

New insight into the aptamer conformation and aptamer/protein interaction by surface-enhanced Raman scattering and multivariate statistical analysis

Wafa Safar
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Andra-Sorina Tatar
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Monica Potara
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Qiqian Liu
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Nadia Djaker
Jolanda Spadavecchia
Weiling Fu
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Sarra Gam Derouich
Nordin Felidj
Simion Astilean

Résumé

We study the interaction between one aptamer and its analyte (the MnSOD protein) by the combination of surface-enhanced Raman scattering and multivariate statistical analysis. We observe the aptamer structure and its evolution during the interaction under different experimental conditions (in air or in buffer). Through the spectral treatment by principal component analysis of a large set of SERS data, we were able to probe the aptamer conformations and orientations relative to the surface assuming that the in-plane nucleoside modes are selectively enhanced. We demonstrate that the aptamer orientation and thus its flexibility rely strongly on the presence of a spacer of 15 thymines and on the experimental conditions with the aptamer lying on the surface in air and standing in the buffer. We reveal for the first time that the interaction with MnSOD induces a large loss of flexibility and freezes the aptamer structure in a single conformation.
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Dates et versions

hal-03369964 , version 1 (23-11-2021)

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Wafa Safar, Andra-Sorina Tatar, Aymeric Leray, Monica Potara, Qiqian Liu, et al.. New insight into the aptamer conformation and aptamer/protein interaction by surface-enhanced Raman scattering and multivariate statistical analysis. Nanoscale, 2021, 13 (29), pp.12443-12453. ⟨10.1039/D1NR02180J⟩. ⟨hal-03369964⟩
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