,
, A use-dependent sodium current modication induced by type I pyrethroid insecticides in honeybee antennal olfactory receptor neurons, Neurotoxicology, vol.32, issue.3, pp.320-330
, Manuscrit d'auteur / Author manuscript Manuscrit d'auteur / Author manuscript Manuscrit d'auteur / Author manuscript Comment citer ce document
,
, A use-dependent sodium current modication induced by type I pyrethroid insecticides in honeybee antennal olfactory receptor neurons, Neurotoxicology, vol.32, issue.3, pp.320-330
, Tail current decay and estimation of the fraction of modified sodium channels by tetramethrin and permethrin in ORNs
, After repolarization from a 3 ms pulse, the pyrethroid-induced tail current slowly returns towards zero
, The decline of tail currents induced by tetramethrin (10 µM) was estimated by measuring the residual tail current at the end of recordings, 130 ms after repolarization (arrow). B, On average, the tetramethrin-induced tail current reached 30% of its initial value (see text). C, The fraction of modified channels with tetramethrin was estimated in individual neurons according to Eqn 4 (see Methods), A
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,
, A use-dependent sodium current modication induced by type I pyrethroid insecticides in honeybee antennal olfactory receptor neurons, Neurotoxicology, vol.32, issue.3, pp.320-330
, Manuscrit d'auteur / Author manuscript Manuscrit d'auteur / Author manuscript Manuscrit d'auteur / Author manuscript Comment citer ce document
,
, A use-dependent sodium current modication induced by type I pyrethroid insecticides in honeybee antennal olfactory receptor neurons, Neurotoxicology, vol.32, issue.3, pp.320-330
, Manuscrit d'auteur / Author manuscript Manuscrit d'auteur / Author manuscript Manuscrit d'auteur / Author manuscript Fig, vol.9
, Superimposed monoexponential curves were fitted to the mean data obtained from tetramethrin and permethrin-exposed ORNs. C, In a majority of pyrethroid-treated neurons, the use-dependent tail current summation was accompanied by an increase in the peak of the sodium current along with stimulations as illustrated in this neuron (lower trace, arrows), whereas the opposite phenomenon was observed (a peak current decrease) in the control solution (same neuron, top trace). D, At 20 nM, the sea anemone toxin ATX-II induces the appearance of a slowly-inactivating component of the honeybee sodium current (top trace). Deactivation is not affected as evidenced by the lack of any tail current either after a single long depolarization or a train of short 3ms depolarizations (35 Hz). E, ATX-II (20 nM) allows for a recruitment of a large quantity of pyrethroid-modified inactivated channels right away after a single short pulse (3 ms, A, Repetitive stimulations (ten 3 ms-pulses at 35 Hz) lead to the progressive use-dependent development of a more sustained and larger pyrethroid-induced tail current