Impact of reducing and oxidizing agents on the infectivity of Q beta phage and the overall structure of its capsid - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Ecology Année : 2016

Impact of reducing and oxidizing agents on the infectivity of Q beta phage and the overall structure of its capsid

Résumé

Q beta phages infect Escherichia coli in the human gut by recognizing F-pili as receptors. Infection therefore occurs under reducing conditions induced by physiological agents (e.g. glutathione) or the intestinal bacterial flora. After excretion in the environment, phage particles are exposed to oxidizing conditions and sometimes disinfection. If inactivation does not occur, the phage may infect new hosts in the human gut through the oral route. During such a life cycle, we demonstrated that, outside the human gut, cysteines of the major protein capsid of Q beta phage form disulfide bonds. Disinfection with NaClO does not allow overoxidation to occur. Such oxidation induces inactivation rather by irreversible damage to the minor proteins. In the presence of glutathione, most disulfide bonds are reduced, which slightly increases the capacity of the phage to infect E. coli in vitro. Such reduction is reversible and barely alters infectivity of the phage. Reduction of all disulfide bonds by dithiothreitol leads to complete capsid destabilization. These data provide new insights into how the phages are impacted by oxidizing-reducing conditions outside their host cell and raises the possibility of the intervention of the redox during life cycle of the phage.Physiological redox conditions modify disulfide bonds of the capsid of Q beta phage without decreasing infectivity, whereas total reduction disrupts the capsid and chlorine oxidation disrupts especially minor proteins.Physiological redox conditions modify disulfide bonds of the capsid of Q beta phage without decreasing infectivity, whereas total reduction disrupts the capsid and chlorine oxidation disrupts especially minor proteins.

Dates et versions

hal-01602397 , version 1 (02-10-2017)

Identifiants

Citer

Pauline Loison, Didier Majou, Éric Gelhaye, Nicolas Boudaud, Christophe Gantzer. Impact of reducing and oxidizing agents on the infectivity of Q beta phage and the overall structure of its capsid. FEMS Microbiology Ecology, 2016, 92 (11), ⟨10.1093/femsec/fiw153⟩. ⟨hal-01602397⟩
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