. Arg, For the 375 NEAA, fractional removals increased (P ? 0.05) with AA infusion for Ala, Asp, Cys, Gln, Gly, 376 Pro and Ser. Fractional hepatic removals of His, Phe, Thr, Ala, Gln and Gly decreased (P ? 377 0.03) or tended to decrease (P ? 0.03) for Arg, Lys and Met with glucose infusion (Table 10)

, Mammary Uptake of Individual AA and AA in Milk. Mammary plasma flow did not

, AA × Glc interaction (P = 0.06) in His mammary uptake: the increment was significant only 383 when glucose was infused (P < 0.01). Among NEAA, mammary uptake of Cys increased

. Arg, P. Met, and T. , As previously 386 mentioned, we had a tendency for an AA × Glc interaction (P = 0.06) for His mammary uptake: 387 glucose infusion was significant only with AA infusion (P < 0.01). Among NEAA

, × Glc interaction (P = 0.09) for Pro mammary uptake: AA infusion increased more Pro

, Mammary fractional removals of individual AA decreased (P ? 0.05, Table, vol.10

, Glu and Pro, tended to decrease (P = 0.06) for Val 395 and increased (P = 0.05) for Cys. On the other hand, glucose infusion increased (P < 0.01) 396 fractional removals of Ile, AA infusion for His, p.25

, Met and Phe) and Tyr, mammary uptake increased with glucose 598 infusion with unchanged VA differences coupled to an increased mammary plasma flow

. Lapierre, Nevertheless, the uptakes were not sufficient to 609 cover the milk output in protein, with Gln being the only NEAA for which the U:O ratio is 610 never lower than 1. With AA infusion, i.e., when AA supply was in excess, decreased U:O 611 ratios of Ala, Glu and Tyr indicate increased mammary de novo synthesis. So, overall, although 612 it is acknowledged that AA infusion increases rate of appearance of glucose, vol.613, 2010.

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. Eaa-n:-sum-of-arg, . His, . Ile, . Leu, . Lys et al.,

A. Group1, Sum of His, Met, Phe and Tyr

. Hmp-n, Sum of His, Met, Phe

A. Group2,

. Bcaa-n,

. Neaa-n:-sum-of-ala, . Asn, . Asp, . Cys, . Gln et al.,

, Ctrl: water infusion; AA CN : 695 g/d of AA mixture with casein profile infusion; E Glc : 1,454 g/d of glucose infusion

A. A. Cn-+-e-glc, combination of AA mixture and glucose infusion

, Probability values correspond to the effect of AA (without vs. with AA infusion); effect of Glc (without vs. with glucose infusion); and interaction between AA and Glc

. Hmp-n, Sum of His, Met and Phe

, PDV catabolism : catabolism across PDV

, Peri catabolism : catabolism across peripheral tissues other than mammary gland

, MG catabolism : catabolism across mammary gland

A. Group2,

. Neaa-n:-sum-of-ala, . Asn, . Asp, . Cys, . Gln et al.,

, Obtained by difference between MP and EAA-N fractions

&. , , vol.10, p.945