Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Dynamic transcriptomic changes of goat abomasal mucosa in response to Haemonchus contortus infection

Abstract : AbstractGastrointestinal nematode (GIN) infections are one of the major constraints for grazing sheep and goat production worldwide. Genetic selection for resistant animals is a promising control strategy. Whole-transcriptome analysis via RNA-sequencing (RNA-seq) provides knowledge of the mechanisms responsible for complex traits such as resistance to GIN infections. In this study, we used RNA-seq to monitor the dynamics of the response of the abomasal mucosa of Creole goat kids infected with Haemonchus contortus by comparing resistant and susceptible genotypes. A total of 8 cannulated kids, 4 susceptible and 4 resistant to GIN, were infected twice with 10 000 L3 H. contortus. During the second infection, abomasal mucosal biopsies were collected at 0, 8, 15 and 35 days post-infection (dpi) from all kids for RNA-seq analysis. The resistant animals showed early activation of biological processes related to the immune response. The top 20 canonical pathways of differentially expressed genes for different comparison showed activation of the immune response through many relevant pathways including the Th1 response. Interestingly, our results showed a simultaneous time series activation of Th2 related genes in resistant compared to susceptible kids.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [40 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-02514818
Déposant : Bmc Bmc <>
Soumis le : lundi 23 mars 2020 - 05:11:24
Dernière modification le : lundi 15 juin 2020 - 10:20:57
Document(s) archivé(s) le : mercredi 24 juin 2020 - 12:58:04

Fichier

13567_2020_Article_768.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Citation

Hadeer M. Aboshady, Nathalie Mandonnet, Yoann Félicité, Julien Hira, Aurore Fourcot, et al.. Dynamic transcriptomic changes of goat abomasal mucosa in response to Haemonchus contortus infection. Veterinary Research, BioMed Central, 2020, 51 (1), pp.1-12. ⟨10.1186/s13567-020-00768-y⟩. ⟨hal-02514818⟩

Partager

Métriques

Consultations de la notice

335

Téléchargements de fichiers

487