New advances on the Brettanomyces bruxellensis biofilm mode of life - Archive ouverte HAL Access content directly
Journal Articles International Journal of Food Microbiology Year : 2020

New advances on the Brettanomyces bruxellensis biofilm mode of life

(1) , (1) , (2) , (3) , (4) , (4) , (1) , (5) , (2) , (3) , (3) , (3)
1
2
3
4
5

Abstract

The wine spoilage yeast Brettanomyces bruxellensis can be found at several steps in the winemaking process due to its resistance to multiple stress conditions. The ability to form biofilm is a potential resistance strategy, although it has been given little attention so far for this yeast. In this work, the capacity to form biofilm and its structure were explored in YPD medium and in wine. Using microsatellite analysis, 65 isolates were discriminated into 5 different genetic groups from which 12 strains were selected. All 12 strains were able to form biofilm in YPD medium on a polystyrene surface. The presence of microcolonies, filamentous cells and extracellular polymeric substances, constituting the structure of the biofilm despite a small thickness, were highlighted using confocal and electronic microscopy. Moreover, different cell morphologies according to genetic groups were highlighted. The capacity to form biofilm in wine was also revealed for two selected strains. The impact of wine on biofilms was demonstrated with firstly considerable biofilm cell release and secondly growth of these released biofilm cells, both in a strain dependent manner. Finally, B. bruxellensis has been newly described as a producer of chlamydospore-like structures in wine, for both planktonic and biofilm lifestyles.
Fichier principal
Vignette du fichier
S0168160519303952.pdf (8.07 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-02537081 , version 1 (21-07-2022)

Licence

Attribution - NonCommercial - CC BY 4.0

Identifiers

Cite

Manon Lebleux, Hany Abdo, Christian Coelho, Louise Basmaciyan, Warren Albertin, et al.. New advances on the Brettanomyces bruxellensis biofilm mode of life. International Journal of Food Microbiology, 2020, 318, pp.1-10. ⟨10.1016/j.ijfoodmicro.2019.108464⟩. ⟨hal-02537081⟩
93 View
11 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More