Skip to Main content Skip to Navigation
Journal articles

Genetic resources in maritime pine (Pinus pinaster Aiton): molecular and quantitative measures of genetic variation and differentiation among maternal lineages

Abstract : Pinus pinaster is a conifer native to western Europe and northern Africa. Following on-going breeding programmes, provenance and progeny trials were established in some of the countries of the species` range (France, Portugal and Spain) and quantitative traits were measured: growth, stem form, survival and pest and disease resistance, amongst others. Populations from the wide range of P. pinaster were recently screened with molecular markers in order to assess their genetic diversity. Data were obtained using allozymes, chloroplast (cpSSRs) and nuclear (nuSSRs) microsatellites and amplified fragment length polymorphisms (AFLPs). Based on mtDNA-RFLP analysis, three maternal lineages (named "western", "eastern" and "Moroccan") were identified and no population showed a mixed composition. In this study, the imprint that differentiation in putatively isolated glacial refugia (identified by the different maternal lineages) might have left on the nuclear genome was analysed using a wide range of molecular markers and adaptive traits. Multivariate ordination of populations based on nuclear molecular markers (allozymes and nuSSRs) showed a clear clustering of provenances sharing a given mtDNA lineage. However, that clustering was found to be less tight when only quantitative traits were investigated. In P. pinaster, the within-population estimates of gene diversity using different traits were not correlated. Therefore, caution is advisable when designing conservation strategies based on molecular marker studies or a limited number of populations. After these results, we recommend a conservation strategy characterised by gene flow consistent with the current population structure, careful seed transfer among maternal lineages (if any), selection of populations for conservation based on the originality of their allelic composition and definition of Management Units (MUs) based on adaptive traits.
Mots-clés : CEMAGREF EFNO
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02583015
Contributor : Migration Irstea Publications Connect in order to contact the contributor
Submitted on : Thursday, May 14, 2020 - 10:05:27 PM
Last modification on : Friday, October 8, 2021 - 11:54:10 AM

Identifiers

Collections

Citation

S.C. Gonzalez Martinez, S. Mariette, M.M. Ribeiro, C. Burban, Annie Raffin, et al.. Genetic resources in maritime pine (Pinus pinaster Aiton): molecular and quantitative measures of genetic variation and differentiation among maternal lineages. Forest Ecology and Management, Elsevier, 2004, 197 (1-3), pp.103-115. ⟨10.1016/j.foreco.2004.05.008⟩. ⟨hal-02583015⟩

Share

Metrics

Record views

44