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Performance of diffusion gradient in thin films to evaluate the toxic fraction of copper to Daphnia Magna

Abstract : This study investigates the relevance of the Diffusion Gradient in Thin films technique (DGT) to measure copper's induced lethality on Daphnia magna in natural water spiked with various organic ligands. Ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA) and glycine were used as artificial organic ligands in controlled solutions of mineral water. With EDTA, DGT measurement makes it possible to predict the toxicity of the mixture because Cu-EDTA complexes are inert, whereas DGT is of no help in case of NTA, because Cu-NTA complexes are fully labile. Cu-glycine complexes appear as partly labile and toxic. Humic acids as well as fresh and aged algae extracts were also used as models for natural dissolved organic matters. All three of them form copper complexes that are not toxic to Daphnia magna and appear as partly labile with open-pored DGT. However, the use of restricted gels in DGT greatly reduces the contribution of labile complexes, at least for humic acids and aged algae copper complexes. DGT with restrictive gels therefore appears to be a powerful tool for measuring bioavailable copper in natural water bodies, especially when the dissolved organic matter is mostly of humic origin. DGT's potential ability to mimic the lability induced by the biological uptake is also discussed.
Mots-clés : DGT
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https://hal.inrae.fr/hal-02583056
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Submitted on : Thursday, May 14, 2020 - 10:06:58 PM
Last modification on : Thursday, February 18, 2021 - 3:06:58 AM

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M.H. Tusseau-Vuillemin, R. Gilbin, Estelle Bakkaus, Jeanne Garric. Performance of diffusion gradient in thin films to evaluate the toxic fraction of copper to Daphnia Magna. Environmental Toxicology and Chemistry, Wiley, 2004, 23 (9), pp.2154-2161. ⟨10.1897/03-202a⟩. ⟨hal-02583056⟩

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