The main objective of the integrated modelling framework (SEAMLESS-IF) is to support analysis of agricultural systems and assessment of impacts related to sustainability and sustainable development. To reach this objective, two test cases, driven by economic (test case 1) or environmental policies (test case 2), are considered to test and improve SEAMLESS-IF. The focus of this deliverable is to describe the major characteristics of environmental policies and agro-ecological technologies to be studied in test case 2. This deliverable will provide the background of knowledge, policies and technologies on which policy scenarios will be defined in Test case 2 (PD 6.2.4, SEAMLESS DOW, 2005). The report is divided into three parts. The first part describes the stakes and the current situation of water use, water pollution and biodiversity in EU and in each of the agricultural regions (as defined in Perez et al., 2005) selected for detailled implementation of the test case in France (The Neste and the Massif Central) and in Poland (Pyrzyce). It further reviews the main difference on water use, pollution and biodiversity, between the north and the south of Europe and between accession and western European countries. The second part of the report details the main European regulations and directives aimed to reduce the use of the water, the water pollution by nitrate and pesticide and to preserve biodiversity. This part of the deliverable is divided in three paragraphs: 1.The overview of directives and regulations to control water use and pollution by describing nitrate directives and pesticide legislation. 2. Birds and habitats directives to preserve biodiversity. 3.Implementation of directives to preserve water quality, water use and biodiversity, in each agricultural region of the test case in France and Poland. The first two points include a discussion on the effect of crop rotation and management, via experiments through the world and in Europe, to reduce water use and pollution by nitrate and pesticide. This part describes also the role of grassland to preserve biodiversity. The last part describes main agro-ecological innovations to preserve water and biodiversity. It is focussed on agro-ecological innovations such as conservation agriculture, agroforestry, organic farming which are known to preserve water and biodiversity.