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Communication Dans Un Congrès Année : 2009

Effect of Triclosan and Diuron pulse on fluvial biofilms

Effets de pulses de triclosan et diuron sur les biofilms de cours d'eau

Résumé

During last decades, personal care products and herbicides consume highly increased and can reach, through diffuse and/or punctual sources, aquatic ecosystems where are detected at low concentration. Among these, Triclosan (TCS) is a broad-spectrum antimicrobial used in products as toothpaste, shampoos, deodorants, skin lotions, and hand soaps. Diuron (DIU) is an herbicide that blocks the electron transfer at the level of photosystem II of photosynthetic organisms. Several studies investigated effects of these compounds on aquatic organisms in a single-specie tests, less studies are focused on the biofilm community response. Nevertheless, no previous studies investigated risks of entrance of both compounds for ecosystem services as auto-depuration capacity of rivers. In order to assess these risks, a mesocosms experiment was performed. 4 wk-old biofilms grown on artificial glass substrata under controlled conditions were exposed to TCS and DIU during 48 h. Phosphorus (P) uptake capacity was measured before and after exposure. Recovery of P uptake capacity was followed during 2 weeks (once per week) after exposure. Bacteria mortality was also measured in each sampling date as well as other structural and functional endpoints (Chl a, Extracellular Peptidase and alkaline Phosphatase activities, live/dead diatoms ratio, photosynthetic efficiency and diatoms growth rate). Samples were also analyzed by SEM microscopy. TCS mainly affected P uptake capacity and bacteria mortality. Inhibition of 71% of P uptake capacity was measured after 48h exposure. 1 week after exposure P uptake capacity partially recovered even if inhibition of 22% was measured and Uptake rate (U, μg cm-2 h-1) of exposed biofilms was still significantly lower than control (p < 0.001). 2 weeks before exposure P uptake capacity recovered (inhibition of 4%). Bacterial mortality increased after 48h exposure (45%) and recovered after 1 week. Moreover, SEM pictures suggests specie-specific effect of TCS on Spyrogira sp. that persisted at week 2. DIU mainly affected diatoms mortality with an increase of 79% after 48h. Diatoms did not recover and mortality resulted still 35% higher than control at week 2. Finally, both TCS and DIU exposure significantly affected diatoms growth rate.

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Dates et versions

hal-02592317 , version 1 (15-05-2020)

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L. Proia, M. Peipoch, Soizic Morin, Anna Romani, Sergi Sabater. Effect of Triclosan and Diuron pulse on fluvial biofilms. XIX Congresso S.It.E, Sep 2009, Bolzano, Italy. pp.1. ⟨hal-02592317⟩

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