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Article Dans Une Revue Journal of Industrial Microbiology and Biotechnology Année : 2009

A Saccharomyces cerevisiae-based bioassay for assessing pesticide toxicity

Mise au point d'un test biologique basé sur la levure Saccharomyces cerevisiae pour évaluer la toxicité des pesticides

Résumé

This study evaluates the toxic effect of three pesticides Azoxystrobin, Cymoxanil, and Diuron) on the yeast Saccharomyces cerevisiae for the development of a new bioassay based on inhibition of S. cerevisiae metabolic activity at the level of adenosine-5-triphosphate (ATP) synthesis, as compared with two different toxicity tests based on inhibition of Daphnia magna mobility (NF EN ISO 6341) and inhibition of Vibrio fisheri activity (NF EN ISO 11348). The S. cerevisiae bioassay is cheaper and 96 times faster than the D. magna toxicity bioassay, but has lower sensitivity. It is as fast as the V. fisheri bioassay and more sensitive. Thus, this new toxicity test can be proposed for rapid detection of pesticide residues in environmental samples as a complement to the more expensive and timeconsuming D. magna toxicity test.

Dates et versions

hal-02594947 , version 1 (15-05-2020)

Identifiants

Citer

Karine Esteve, Christian Poupot, P. Dabert, M. Mietton Peuchot, Vladan Milisic. A Saccharomyces cerevisiae-based bioassay for assessing pesticide toxicity. Journal of Industrial Microbiology and Biotechnology, 2009, 36 (12), pp.1529-1534. ⟨10.1007/s10295-009-0649-1⟩. ⟨hal-02594947⟩

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