Skip to Main content Skip to Navigation
Poster communications

Development of q-PCR approaches to assess water quality: Effects of direct cadmium exposure on gene expression of the diatom Eolimna minima

Abstract : In regard to the degradation of water quality since the last decades, appropriate diagnostic tools have been implemented. In France, water agencies in collaboration with Cemagref have developed a diatom index to estimate global water quality. The Biological Diatom Index (BDI, Coste et al. 2009) is routinely used for monitoring applications in European territories and has been standardized (NF T 90-354). Nevertheless, indices currently used for water quality assessment don't take in consideration metal contamination in their conception, despite the high bioaccumulation and impact shown on periphytic diatom communities (da Silva et al., 2009, Cunningham et al., 2005, Feurtet-Mazel et al., 2003). Moreover these methods are time consuming and require important taxonomic knowledge. In this context development of q-PCR approaches are of particular interest. In our study we have developed a new RNA extraction method for diatoms, 8 genes of interest for the diatom Eolimna minima have been selected, sequenced and deposited in the GenBank. The responses of the q-PCR tools developed have been tested after Cd exposure on Eolimna minima. Bioaccumulation and population kinetics were also followed.
Document type :
Poster communications
Complete list of metadata

https://hal.inrae.fr/hal-02595066
Contributor : Migration Irstea Publications <>
Submitted on : Friday, May 15, 2020 - 7:37:11 PM
Last modification on : Thursday, June 3, 2021 - 5:34:03 PM

File

pub00031883.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : hal-02595066, version 1
  • IRSTEA : PUB00031883

Collections

Citation

S. Kim Tiam, A. Feurtet Mazel, François Delmas, Nicolas Mazzella, Soizic Morin, et al.. Development of q-PCR approaches to assess water quality: Effects of direct cadmium exposure on gene expression of the diatom Eolimna minima. 21st SETAC Europe Annual Meeting, May 2011, Milan, Italy. pp.1, 2011. ⟨hal-02595066⟩

Share

Metrics

Record views

42

Files downloads

30