Grab sampling may be scarcely adequate to evaluate the impact on surface waters of pesticide used in agriculture because of its poor spatiotemporal representativeness compared to the high variability of the contamination patterns. Indeed, it is still not easy to link the effect of agricultural practice changes to water quality at the catchment scale. Furthermore, biological indicators may also be useful in addition to pesticide concentration determination to evaluate their impact on ecosystems. In this way, the contribution of new integrative tools has been evaluated. Passive samplers and biological indicators have been applied together at the catchment scale so as to identify their coherence and complementarity. The tested chemical tools are the Silicone Rod (SR) dedicated to hydrophobic to moderately hydrophilic compounds. In total, 28 pesticides (14 herbicides, 6 fungicides and 6 insecticides) were analyzed as well as potential confusing parameters (metal(oïd)s, nutrients, temperature). In parallel the biological approach consisted in an in situ study of i) leaf litter decomposition processes, which are mediated by the combined action of microbial and invertebrate communities and of ii) the measurement of fungal biomass within leaf-associated microbial communities. These tools have been applied during June 2014 and June 2015 (corresponding to a period of pesticides application on vineyard) in Ardières river. The Ardières basin situated in the Beaujolais region (France) is submitted to viticulture pressure and a marked pesticide contamination has already been highlighted. Three measurement stations have been chosen along the contamination gradient from upstream to downstream. Results showed the high coherence of chemical and biological tools responses. All of them were able to reflect the spatial contamination gradients as well as the specific contamination and impact associated. Thus, decomposition rates and fungal biomass decreased from up to downstream, in relation to the observed increase in fungicides and organophosphorous insecticides concentrations. This biological impact was observed during the two studied experimental years, while pesticide contamination decreased in 2015 compared to 2014. Finally, their sensitivity, reactivity and integration capacity make these tools relevant and promising to assess agricultural pesticide impact on surface water in addition to grab sampling.