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Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity

Abstract : the pectin methylesterase action is usually studied in a homogeneous aqueous medium in the presence of a large excess of soluble substrate and water. However in the cell wall, the water content is much lower, the substrate is cross-linked with itself or with other polymers, and the enzyme has to diffuse through the solid matrix before catalysing the linkage breakdown. As plant primary cell walls can be considered as cellulose-reinforced hydrogels, this study investigated the diffusion of a fungal pectin methylesterase in pectin/cellulose gels used as cell wall-mimicking matrix to understand the impact of this matrix and its (micro) structure on the enzyme’s diffusion within it. The enzyme mobility was followed by synchrotron microscopy thanks to its auto-fluorescence after deep-UV excitation. Time-lapse imaging and quantification of intensity signal by image analysis revealed that the diffusion of the enzyme was impacted by at least two criteria: (i) only the active enzyme was able to diffuse, showing that the mobility was related to the catalytic ability, and (ii) the diffusion was improved by the presence of cellulose in the ge
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Déposant : Migration Prodinra <>
Soumis le : lundi 25 mai 2020 - 15:00:33
Dernière modification le : mardi 7 juillet 2020 - 10:55:22

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Estelle Bonnin, Camille Alvarado, Marie-Jeanne Crepeau, Brigitte Bouchet, Catherine Garnier, et al.. Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity. Scientific Reports, Nature Publishing Group, 2019, 9 (12551), pp.1-10. ⟨10.1038/s41598-019-49108-x⟩. ⟨hal-02618559⟩

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