HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Microarray and mass spectrometry-based methodology for lipid profiling of tissues and cell cultures

Abstract : The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers in the context of different diseases and as indicators of the cell's homeostasis. However, further advances are required to unveil the complex relationships between lipid classes and lipid species with proteins. Here, we present a new methodology that combines microarrays with mass spectrometry to obtain the lipid fingerprint of samples of a different nature in a standardized and fast way, with minimal sample consumption. As a proof of concept, we use the methodology to obtain the lipid fingerprint of 20 rat tissues and to create a lipid library for tissue classification. Then, we combine those results with immunohistochemistry and enzymatic assays to unveil the relationship between some lipid species and two enzymes. Finally, we demonstrate the performance of the methodology to explore changes in lipid composition of the nucleus accumbens from mice subjected to two lipid diets.
Complete list of metadata

https://hal.inrae.fr/hal-02618866
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Monday, May 25, 2020 - 3:37:52 PM
Last modification on : Monday, December 13, 2021 - 1:54:20 PM

Identifiers

Collections

Citation

Roberto Fernandez, Jone Garate, Tarson Tolentino-Cortez, Ainara Herraiz, Laura Lombardero, et al.. Microarray and mass spectrometry-based methodology for lipid profiling of tissues and cell cultures. Analytical Chemistry, American Chemical Society, 2019, 91 (24), pp.15967-15973. ⟨10.1021/acs.analchem.9b04529⟩. ⟨hal-02618866⟩

Share

Metrics

Record views

5