G. Romay, M. Pitrat, H. Lecoq, C. Wipf-scheibel, P. Millot et al., Resistance against melon chlorotic mosaic virus and tomato leaf curl New Delhi virus in melon, Plant Disease, vol.103, issue.11, pp.2913-2919, 2019.
URL : https://hal.archives-ouvertes.fr/hal-02619468

, PI 124112 and PI 282448 did not show any, Accessions IC-274014, WM 7, vol.9

, Védrantais exhibited severe mosaic symptoms, stunting and enations when inoculated 203 with MeCMV and the virus was clearly detected by ELISA or PCR. IC-274014 exhibited no 204 symptom and the virus was not detected. The F 1 (IC-274014 ? Védrantais) exhibited 205 systemic chlorotic spots and the virus was detected in apical leaves (Figure 1), indicating a 206 recessive genetic control for virus infection

. Bc-s-was-fully-susceptible, All the BC S plants exhibited a range of symptoms: severe 208 mosaic and stunting, mild mosaic or mottle or systemic chlorotic spots and the virus was 209 detected by ELISA in all the plants. In the BC R progeny, some plants had no symptoms but 210 the majority exhibited symptoms varying from systemic chlorotic spots to mottle; the virus 211 was not detected by ELISA and/or PCR in 29/95 plants (Table 2). This observed segregation 212 fitted a 3 susceptible : 1 resistant ratio expected for two independent recessive genes

, In the F 2 progeny, all types of symptoms were observed (mosaic, mottle, a majority with 215 systemic chlorotic spots) and some plants had no visible symptoms. The virus was detected Comment citer ce document

. Romay, In several accessions, intermediate 336 resistance mechanisms appeared related to a "recovery" phenotype, as observed after 337 melon or watermelon infection with CuLCrV (Hagen et al. 2008a). This suggests that the 338 resistance could be related in these cases to RNA silencing, 2015.

. Hagen, although the actual mechanism remains unknown, 2008.

, Accession IC-274014 was resistant (no symptoms and no virus detection by ELISA or PCR)

M. To, Different types of symptoms were observed in the F 1 , F 2 and BC progenies 342 between IC-274014 and the susceptible cultivar Védrantais: severe mosaic, mottle, systemic 343 chlorotic spots. Inheritance of these different types of symptoms was difficult to analyse in 344 terms of genetic control. The F 1 exhibited systemic chlorotic spots which were not observed 345 in the parents. In the F 2 or BC progenies, "recovery" phenotypes were often observed, with 346 much higher symptom severity in the old leaves than in young ones. In plants with the 347 different types of symptoms, the virus was always detected by ELISA or PCR

, Resistance of IC-274014 to ToLCNDV could be controlled by one recessive and two to both viruses, even if the hypothesis of genes belonging to the same clusters 355 cannot be completely ruled out, clusters of resistance genes being present in melon

. Mas, We propose the names begomovirus resistance-1and, 2012.

, Begomovirus resistance-2 for these genes (symbols bgm-1 and Bgm-2) corresponding Comment citer ce document

. Romay, Plant Disease

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, Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota, PLoS Genet, vol.486, p.10

H. Lecoq and M. Pitrat, Strains of zucchini yellow mosaic virus in muskmelon 488 (Cucumis melo L.), J. Phytopathol, vol.111, pp.165-173, 1984.

H. Lecoq, G. Wilser, and M. Pitrat, Cucurbit viruses: the classics and the emerging. 490 Proceedings of Cucurbitaceae 98, ASHS & U.S. Dep. Agric.-ARS, pp.126-142, 1998.

C. Lopez, M. Ferriol, and M. B. Pico, Mechanical transmission of Tomato leaf curl New 492, 2015.

, Delhi virus to cucurbit germplasm: selection of tolerance sources in Cucumis melo, Euphytica, vol.493, pp.679-691

C. Mallor, J. M. Alvarez, and M. Luis-arteaga, A resistance to systemic symptom 495 expression of melon necrotic spot virus in melon, J. American Soc.Hort. Sci, vol.128, pp.541-547, 2003.

J. D. Mccreight, H. Liu, and T. A. Turini, Genetic resistance to Cucurbit Leaf 497 Crumple Virus in melon, HortScience, vol.43, pp.122-126, 2008.

F. J. Morales and A. I. Niessen, Comparative responses of selected Phaseolus vulgaris 499 germ plasm inoculated artificially and naturally with bean golden mosaic virus, Plant Dis. Comment citer ce document, 1988.

. Romay, MNSV: melon necrotic spot virus (carmovirus), 549 MRMV: melon rugose mosaic virus (tymovirus), PRSV: papaya ringspot virus (potyvirus), WMV: watermelon mosaic virus 551 (potyvirus), WmCSV: watermelon chlorotic stunt virus (begomovirus), ZYMV: zucchini yellow 552 mosaic virus (potyvirus). Begomoviruses (CuLCrV, MeCMV, ToLCNDV, WmCSV) are, vol.550

, Comment citer ce document

. Romay, Symptoms of MeCMV: curling, yellowing and stunting in the susceptible cv, vol.588

, Védrantais (a); no symptom and no virus multiplication in the resistant accession IC-274014

, Comment citer ce document

. Romay, Plant Disease

, Supplementary Table S1: Observed number of plants susceptible and resistant to MeCMV in 594 the F 4 (IC-274014 ? Védrantais) progenies after selection of the F 2 for resistance to this 595 virus, and adjusted number for the same size of F 4 plants for the different, vol.3

, Supplementary Table S3: Expected vs. observed frequency for susceptible vs. resistant plants 601 to ToLCNDV in the F 3 and F 4 progenies derived from F 2 (IC-274014 ? Védrantais) resistant to

, MeCMV, and probability of the chi-square under different hypotheses of linkage (or identity) 603 of genes involved in resistance to MeCMV (a, b and C) and to ToLCNDV (x, Y and Z)

, Supplementary table S4: behaviour of Védrantais, IC-274014, F 1 and F 4

, ?Védrantais) progenies towards CABYV and ZYMV, in relation to their susceptibility to the

, Comment citer ce document

. Romay, Plant Disease F 4 progenies obtained without further selection for virus resistance were calculated based on these frequencies in the F 2 plants