CalY is a major virulence factor and a biofilm matrix protein - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Molecular Microbiology Year : 2019

CalY is a major virulence factor and a biofilm matrix protein

Abstract

The extracellular biofilm matrix often contains a network of amyloid fibers which, in the human opportunistic pathogen Bacillus cereus, includes the two homologous proteins TasA and CalY. We show here, in the closely related entomopathogenic species Bacillus thuringiensis, that CalY also displays a second function. In the early stationary phase of planktonic cultures, CalY was located at the bacterial cell-surface, as shown by immunodetection. Deletion of calY revealed that this protein plays a major role in adhesion to HeLa epithelial cells, to the insect Galleria mellonella hemocytes and in the bacterial virulence against larvae of this insect, suggesting that CalY is a cell-surface adhesin. In mid-stationary phase and in biofilms, the location of CalY shifted from the cell surface to the extracellular medium, where it was found as fibers. The transcription study and the deletion of sipW suggested that CalY change of location is due to a delayed activity of the SipW signal peptidase. Using purified CalY, we found that the protein polymerization occurred only in the presence of cell-surface components. CalY is, therefore, a bifunctional protein, which switches from a cell-surface adhesin activity in early stationary phase, to the production of fibers in mid-stationary phase and in biofilms.
Fichier principal
Vignette du fichier
CalY_paper_prereview_manuscript_version_til_CRISTIN.pdf (1.73 Mo) Télécharger le fichier
CalY_221118.pdf (2.59 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Origin Files produced by the author(s)

Dates and versions

hal-02620254 , version 1 (30-11-2023)
hal-02620254 , version 2 (08-12-2023)

Identifiers

Cite

Thomas Candela, Annette Fagerlund, Christophe Buisson, Nathalie Gilois, Anne‐brit Kolstø, et al.. CalY is a major virulence factor and a biofilm matrix protein. Molecular Microbiology, 2019, 111 (6), pp.1416-1429. ⟨10.1111/mmi.14184⟩. ⟨hal-02620254v2⟩
110 View
21 Download

Altmetric

Share

More