Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in <em>Arabidopsis</em> - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 2019

Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis

Résumé

Spontaneous post-transcriptional silencing of sense transgenes (S-PTGS) is established in each generation and is accompanied by DNA methylation, but the pathway of PTGS-dependent DNA methylation is unknown and so is its role. Here we show that CHH and CHG methylation coincides spatially and temporally with RDR6-dependent products derived from the central and 3' regions of the coding sequence, and requires the components of the RNA-directed DNA methylation (RdDM) pathway NRPE1, DRD1 and DRM2, but not CLSY1, NRPD1, RDR2 or DCL3, suggesting that RDR6-dependent products, namely long dsRNAs and/or siRNAs, trigger PTGS-dependent DNA methylation. Nevertheless, none of these RdDM components are required to establish S-PTGS or produce a systemic silencing signal. Moreover, preventing de novo DNA methylation in non-silenced transgenic tissues grafted onto homologous silenced tissues does not inhibit the triggering of PTGS. Overall, these data indicate that gene body DNA methylation is a consequence, not a cause, of PTGS, and rule out the hypothesis that a PTGS-associated DNA methylation signal is transmitted independent of a PTGS signal.
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hal-02620303 , version 1 (25-05-2020)

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Paternité - Pas d'utilisation commerciale

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Christelle Taochy, Agnès Yu, Nicolas Bouche, Nathalie Bouteiller, Taline Elmayan, et al.. Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis. Nucleic Acids Research, 2019, 47 (17), pp.9104-9114. ⟨10.1093/nar/gkz636⟩. ⟨hal-02620303⟩
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