Diversity of bacterial communities in French chicken cuts stored under modified atmosphere packaging - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Food Microbiology Année : 2018

Diversity of bacterial communities in French chicken cuts stored under modified atmosphere packaging

Résumé

Poultry meat, the second most consumed meat in France, is commercialized mainly as portions of chicken cuts with various quality labels, stored under various modified atmosphere packaging (MAP), with shelf-life ranging from 9 to 17 days. We used 16S rDNA pyrosequencing to describe microbiota of chicken legs. Ten samples representing a wide diversity of labels and MAP available on the market were collected from local supermarkets and stored at 4 C. Microbiota were collected, total DNA was extracted, and V1-V3 fragment of 16S rRNA genes were ampli fi ed and sequenced. For data analysis several pipelines were compared. The Qiime pipeline was chosen to cluster reads and we used a database previously developed for a meat and fi sh microbial ecology study. Variability between samples was observed and a listing of bacteria present on chicken meat was established. The structure of the bacterial communities were compared with traditional cultural methods and validated with quantitative real time PCR. Bro-chothrix thermosphacta,Pseudomonas sp., and Carnobacterium sp. were dominant and the nature of the gas used for packaging in fl uenced the relative abundance of each suggesting a MAP gas composition dependent competition between these species. We also noticed that slaughterhouse environment may in fl uence the nature of the contaminants.
Fichier non déposé

Dates et versions

hal-02621918 , version 1 (26-05-2020)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

Citer

Amélie Rouger, Nicolas Moriceau, Hervé Prévost, Benoit Remenant, Monique Zagorec. Diversity of bacterial communities in French chicken cuts stored under modified atmosphere packaging. Food Microbiology, 2018, 70, pp.7-16. ⟨10.1016/j.fm.2017.08.013⟩. ⟨hal-02621918⟩
9 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More