N. Pardi, M. J. Hogan, F. W. Porter, and D. Weissman, mRNA vaccines -a new era in vaccinology, Nat. Rev. Drug Discov, vol.17, pp.261-279, 2018.

S. Rauch, E. Jasny, K. E. Schmidt, and B. Petsch, New vaccine technologies to combat outbreak situations, Front. Immunol, vol.9, 1963.

J. J. Suschak, J. A. Williams, and C. S. Schmaljohn, Advancements in DNA vaccine vectors, non-mechanical delivery methods, and molecular adjuvants to increase immunogenicity, Hum Vaccin Immunother, vol.13, pp.2837-2848, 2017.

D. Hobernik and M. Bros, DNA vaccines-How far from clinical use?, Int. J. Mol. Sci, vol.19, 2018.

T. J. Kim, H. T. Jin, S. Y. Hur, H. G. Yang, Y. B. Seo et al., Sung, Clearance of persistent HPV infection and cervical lesion by therapeutic DNA vaccine in CIN3 patients, Nat. Commun, vol.5, p.5317, 2014.

M. T. Van-diepen, R. Chapman, N. Douglass, S. Galant, P. L. Moore et al., Prime boost immunisations with DNA, MVA and protein-based vaccines elicit robust HIV-1, Tier 2 neutralizing antibodies against the CAP256 superinfecting virus, J. Virol, vol.93, 2019.

P. Tebas, C. C. Roberts, K. Muthumani, E. L. Reuschel, S. B. Kudchodkar et al., Safety and immunogenicity of an anti-Zika virus DNA vaccine -preliminary report, N. Engl. J. Med, 2017.

C. L. Trimble, M. P. Morrow, K. A. Kraynyak, X. Shen, M. Dallas et al., Safety, efficacy, and immunogenicity of VGX-3100, a therapeutic synthetic DNA vaccine targeting human papillomavirus 16 and 18 E6 and E7 proteins for cervical intraepithelial neoplasia 2/3: a randomised, double-blind, Lancet, vol.386, pp.2078-2088, 2015.

C. Collins, N. Lorenzen, and B. Collet, DNA vaccination for finfish aquaculture, Fish Shellfish Immunol, vol.85, pp.106-125, 2018.
URL : https://hal.archives-ouvertes.fr/hal-02624534

J. S. Tregoning and E. Kinnear, Using plasmids as DNA vaccines for infectious diseases, Microbiol Spectr, vol.2, 2014.

J. Lloyd and J. Cheyne, The origins of the vaccine cold chain and a glimpse of the future, Vaccine, vol.35, pp.2115-2120, 2017.

F. Saade and N. Petrovsky, Technologies for enhanced efficacy of DNA vaccines, Expert Rev Vaccines, vol.11, pp.189-209, 2012.

A. Gothelf and J. Gehl, What you always needed to know about electroporation based DNA vaccines, Hum Vaccin Immunother, vol.8, pp.1694-1702, 2012.

K. Schultheis, T. R. Smith, W. B. Kiosses, K. A. Kraynyak, A. Wong et al., Delineating the cell death mechanisms associated with skin electroporation, Hum Gene Ther Methods, vol.29, pp.177-188, 2018.

C. Y. Calvet, F. M. Andre, and L. M. Mir, Dual therapeutic benefit of electroporationmediated DNA vaccination in vivo: enhanced gene transfer and adjuvant activity, Oncoimmunology, vol.3, p.28540, 2014.

S. Babiuk, M. E. Baca-estrada, M. Foldvari, D. M. Middleton, D. Rabussay et al., Increased gene expression and inflammatory cell infiltration caused by electroporation are both important for improving the efficacy of DNA vaccines, J. Biotechnol, vol.110, pp.1-10, 2004.

B. Todorova, L. Adam, S. Culina, R. Boisgard, F. Martinon et al., Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques, Sci. Rep, vol.7, p.4122, 2017.
URL : https://hal.archives-ouvertes.fr/inserm-02437890

J. A. Mikszta, J. B. Alarcon, J. M. Brittingham, D. E. Sutter, R. J. Pettis et al., Improved genetic immunization via micromechanical disruption of skin-barrier function and targeted epidermal delivery, Nat. Med, vol.8, pp.415-419, 2002.

H. S. Gill, J. Soderholm, M. R. Prausnitz, and M. Sallberg, Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine, Gene Ther, vol.17, pp.811-814, 2010.

S. Marshall, L. J. Sahm, and A. C. Moore, The success of microneedle-mediated vaccine delivery into skin, Hum Vaccin Immunother, vol.12, pp.2975-2983, 2016.

G. Cole, A. A. Ali, C. M. Mccrudden, J. W. Mcbride, J. Mccaffrey et al., DNA vaccination for cervical cancer: strategic optimisation of RALA mediated gene delivery from a biodegradable microneedle system, Eur. J. Pharm. Biopharm, vol.127, pp.288-297, 2018.

J. M. Arya, K. Dewitt, M. Scott-garrard, Y. W. Chiang, and M. R. Prausnitz, Rabies vaccination in dogs using a dissolving microneedle patch, J. Control. Release, vol.239, pp.19-26, 2016.

Y. Ma, W. Tao, S. J. Krebs, W. F. Sutton, N. L. Haigwood et al., Vaccine delivery to the oral cavity using coated microneedles induces systemic and mucosal immunity, Pharm. Res, vol.31, pp.2393-2403, 2014.

M. G. Mcgrath, S. Vucen, A. Vrdoljak, A. Kelly, C. O'mahony et al., Production of dissolvable microneedles using an atomised spray process: effect of microneedle composition on skin penetration, Eur. J. Pharm. Biopharm, vol.86, pp.200-211, 2014.

M. Dul, M. Stefanidou, P. Porta, J. Serve, C. O'mahony et al., Hydrodynamic gene delivery in human skin using a hollow microneedle device, J. Control. Release, vol.265, pp.120-131, 2017.
URL : https://hal.archives-ouvertes.fr/hal-01764681

Y. Qiu, L. Guo, S. Zhang, B. Xu, Y. Gao et al., DNAbased vaccination against hepatitis B virus using dissolving microneedle arrays adjuvanted by cationic liposomes and CpG ODN, Drug Deliv, vol.23, pp.2391-2398, 2016.

A. Vrdoljak, E. A. Allen, F. Ferrara, N. J. Temperton, A. M. Crean et al., Induction of broad immunity by thermostabilised vaccines incorporated in dissolvable microneedles using novel fabrication methods, J. Control. Release, vol.225, pp.192-204, 2016.

G. A. Simon and H. I. Maibach, The pig as an experimental animal model of percutaneous permeation in man: qualitative and quantitative observations-an overview, Skin Pharmacol. Appl. Ski. Physiol, vol.13, pp.229-234, 2000.

F. Marquet, T. P. Vu-manh, P. Maisonnasse, J. Elhmouzi-younes, C. Urien et al., Pig skin includes dendritic cell subsets transcriptomically related to human CD1a and CD14 dendritic cells presenting different migrating behaviors and T cell activation capacities, J. Immunol, vol.193, pp.5883-5893, 2014.
URL : https://hal.archives-ouvertes.fr/hal-01194094

J. K. Lunney, Y. Fang, A. Ladinig, N. Chen, Y. Li et al., Porcine reproductive and respiratory syndrome virus (PRRSV): pathogenesis and interaction with the immune system, Annu. Rev. Anim. Biosci, vol.4, pp.129-154, 2016.

A. Kumar, P. Wonganan, M. A. Sandoval, X. Li, S. Zhu et al., Microneedle-mediated transcutaneous immunization with plasmid DNA coated on cationic PLGA nanoparticles, J. Control. Release, vol.163, pp.230-239, 2012.

C. M. Barbon, L. Baker, C. Lajoie, U. Ramstedt, M. L. Hedley et al., In vivo electroporation enhances the potency of poly-lactide co-glycolide (PLG) plasmid DNA immunization, Vaccine, vol.28, pp.7852-7864, 2010.

B. Delmas, J. Gelfi, R. L'haridon, L. K. Vogel, H. Sjostrom et al., Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV, Nature, vol.357, pp.417-420, 1992.

C. Somasundaram, H. Takamatsu, C. Andreoni, J. C. Audonnet, L. Fischer et al., Enhanced protective response and immuno-adjuvant effects of porcine GM-CSF on DNA vaccination of pigs against Aujeszky's disease virus, Vet. Immunol. Immunopathol, vol.70, pp.277-287, 1999.

S. Subramaniam, P. Pineyro, D. Tian, C. Overend, D. M. Yugo et al., In vivo targeting of porcine reproductive and respiratory syndrome virus antigen through porcine DC-SIGN to dendritic cells elicits antigen-specific CD4T cell immunity in pigs, Vaccine, vol.32, pp.6768-6775, 2014.

C. Bernelin-cottet, C. Deloizy, O. Stanek, C. Barc, E. Bouguyon et al., A universal influenza vaccine can Lead to disease exacerbation or viral control depending on delivery strategies, Front. Immunol, vol.7, p.641, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01569908

T. Chrun, S. Lacote, C. Urien, L. Jouneau, C. Barc et al., A Rift Valley fever virus Gn ectodomain-based DNA vaccine induces a partial protection not improved by APC targeting, NPJ Vaccines, vol.3, p.14, 2018.
URL : https://hal.archives-ouvertes.fr/hal-02629280

P. Maisonnasse, E. Bouguyon, G. Piton, A. Ezquerra, C. Urien et al., The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model, Mucosal Immunol, vol.9, pp.835-849, 2016.
URL : https://hal.archives-ouvertes.fr/hal-02394068

G. Grodeland, A. B. Fredriksen, G. A. Loset, E. Vikse, L. Fugger et al., Antigen targeting to human HLA class II molecules increases efficacy of DNA vaccination, J. Immunol, vol.197, pp.3575-3585, 2016.

A. Vrdoljak, M. G. Mcgrath, J. B. Carey, S. J. Draper, A. V. Hill et al., Coated microneedle arrays for transcutaneous delivery of live virus vaccines, J. Control. Release, vol.159, pp.34-42, 2012.

G. Yan, N. Arelly, N. Farhan, S. Lobo, and H. Li, Enhancing DNA delivery into the skin with a motorized microneedle device, Eur. J. Pharm. Sci, vol.52, pp.215-222, 2014.

F. Marquet, M. Bonneau, F. Pascale, C. Urien, C. Kang et al., Characterization of dendritic cells subpopulations in skin and afferent lymph in the swine model, PLoS One, vol.6, p.16320, 2011.

G. Auray, I. Keller, S. Python, M. Gerber, R. Bruggmann et al., Characterization and Transcriptomic analysis of porcine blood conventional and Plasmacytoid dendritic cells reveals striking species-specific differences, J. Immunol, vol.197, pp.4791-4806, 2016.

T. Granot, T. Senda, D. J. Carpenter, N. Matsuoka, J. Weiner et al., Dendritic cells display subset and tissue-specific maturation dynamics over human life, Immunity, vol.46, pp.504-515, 2017.

M. Ghosh, B. Mcauliffe, J. Subramani, S. Basu, and L. H. Shapiro, CD13 regulates dendritic cell cross-presentation and T cell responses by inhibiting receptor-mediated antigen uptake, J. Immunol, vol.188, pp.5489-5499, 2012.

S. Subramaniam, P. Pineyro, R. J. Derscheid, D. M. Madson, D. R. Magstadt et al., Dendritic cell-targeted porcine reproductive and respiratory syndrome virus (PRRSV) antigens adjuvanted with polyinosinic-polycytidylic acid (poly (I:C)) induced non-protective immune responses against heterologous type 2 PRRSV challenge in pigs, Vet. Immunol. Immunopathol, vol.190, pp.18-25, 2017.

H. Mokhtar, M. Eck, S. B. Morgan, S. E. Essler, J. P. Frossard et al., Proteome-wide screening of the European porcine reproductive and respiratory syndrome virus reveals a broad range of T cell antigen reactivity, Vaccine, vol.32, pp.6828-6837, 2014.

N. C. Ganderup, W. Harvey, J. T. Mortensen, and W. Harrouk, The minipig as nonrodent species in toxicology-where are we now?, Int. J. Toxicol, vol.31, pp.507-528, 2012.

A. K. Andrianov, D. P. Decollibus, H. A. Gillis, H. H. Kha, A. Marin et al., Poly[di(carboxylatophenoxy)phosphazene] is a potent adjuvant for intradermal immunization, Proc. Natl. Acad. Sci. U. S. A, vol.106, pp.18936-18941, 2009.

A. Summerfield, F. Meurens, and M. E. Ricklin, The immunology of the porcine skin and its value as a model for human skin, Mol. Immunol, vol.66, pp.14-21, 2015.

K. H. Mair, C. Sedlak, T. Kaser, A. Pasternak, B. Levast et al., The porcine innate immune system: an update, Dev. Comp. Immunol, vol.45, pp.321-343, 2014.

H. D. Dawson, A. D. Smith, C. Chen, and J. F. Urban, An in-depth comparison of the porcine, murine and human inflammasomes; lessons from the porcine genome and transcriptome, Vet. Microbiol, vol.202, pp.2-15, 2017.

H. D. Dawson, C. Chen, B. Gaynor, J. Shao, and J. F. Urban, The porcine translational research database: a manually curated, genomics and proteomics-based research resource, BMC Genomics, vol.18, p.643, 2017.

M. A. Groenen, A. L. Archibald, H. Uenishi, C. K. Tuggle, Y. Takeuchi et al.,

L. A. Kim, M. Frantz, H. Caccamo, B. L. Ahn, A. Aken et al., Analyses of pig genomes provide insight into porcine demography and evolution, pp.393-398, 2012.
URL : https://hal.archives-ouvertes.fr/cea-00880676

R. Kapetanovic, L. Fairbairn, D. Beraldi, D. P. Sester, A. L. Archibald et al., Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide, J. Immunol, vol.188, pp.3382-3394, 2012.

L. Fairbairn, R. Kapetanovic, D. P. Sester, and D. A. Hume, The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and disease, J. Leukoc. Biol, vol.89, pp.855-871, 2011.

T. W. Prow, X. Chen, N. A. Prow, G. J. Fernando, C. S. Tan et al., Nanopatch-targeted skin vaccination against West Nile Virus and Chikungunya virus in mice, Small, vol.6, pp.1776-1784, 2010.

T. R. Smith, K. Schultheis, W. B. Kiosses, D. H. Amante, J. M. Mendoza et al., DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response, Mol. Ther. Methods Clin. Dev, vol.1, p.14054, 2014.

D. H. Amante, T. R. Smith, J. M. Mendoza, K. Schultheis, J. R. Mccoy et al., Skin transfection patterns and expression kinetics of electroporation-enhanced plasmid delivery using the CELLECTRA-3P, a portable next-generation dermal electroporation device, Hum Gene Ther Methods, vol.26, pp.134-146, 2015.

J. Mccaffrey, C. M. Mccrudden, A. A. Ali, A. S. Massey, J. W. Mcbride et al., Transcending epithelial and intracellular biological barriers; a prototype DNA delivery device, J. Control. Release, vol.226, pp.238-247, 2016.

M. Guilliams and L. Van-de-laar, A Hitchhiker's guide to myeloid cell subsets: practical implementation of a novel mononuclear phagocyte classification system, Front. Immunol, vol.6, p.406, 2015.

A. C. Villani, R. Satija, G. Reynolds, S. Sarkizova, K. Shekhar et al., Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors, vol.356, 2017.

M. Otsuka, G. Egawa, and K. Kabashima, Uncovering the mysteries of Langerhans cells, inflammatory dendritic epidermal cells, and monocyte-derived Langerhans cell-like cells in the epidermis, Front. Immunol, vol.9, p.1768, 2018.

T. L. Tang-huau and E. Segura, Human in vivo-differentiated monocyte-derived dendritic cells, Semin. Cell Dev. Biol, vol.86, pp.44-49, 2018.
URL : https://hal.archives-ouvertes.fr/hal-02079402

N. Munoz-wolf and E. C. Lavelle, A guide to IL-1 family cytokines in adjuvanticity, FEBS J, vol.285, pp.2377-2401, 2018.

J. B. Carey, F. E. Pearson, A. Vrdoljak, M. G. Mcgrath, A. M. Crean et al., Microneedle array design determines the induction of protective memory CD8+ T cell responses induced by a recombinant live malaria vaccine in mice, PLoS One, vol.6, p.22442, 2011.

J. B. Carey, A. Vrdoljak, C. O'mahony, A. V. Hill, S. J. Draper et al., Microneedle-mediated immunization of an adenovirus-based malaria vaccine enhances antigen-specific antibody immunity and reduces anti-vector responses compared to the intradermal route, Sci. Rep, vol.4, p.6154, 2014.

D. Poirier, F. Renaud, V. Dewar, L. Strodiot, F. Wauters et al., Hepatitis B surface antigen incorporated in dissolvable microneedle array patch is antigenic and thermostable, Biomaterials, vol.145, pp.256-265, 2017.

J. Wang, B. Li, and M. X. Wu, Effective and lesion-free cutaneous influenza vaccination, Proc. Natl. Acad. Sci. U. S. A, vol.112, pp.5005-5010, 2015.

G. J. Fernando, J. Hickling, C. M. Flores, P. Griffin, C. D. Anderson et al., Safety, tolerability, acceptability and immunogenicity of an influenza vaccine delivered to human skin by a novel high-density microprojection array patch (Nanopatch), Vaccine, vol.36, pp.3779-3788, 2018.

M. P. Schon and M. Schon, Imiquimod: mode of action, Br. J. Dermatol, vol.157, issue.2, pp.8-13, 2007.

W. , Adjuvants for porcine reproductive and respiratory syndrome virus vaccines, Vet. Immunol. Immunopathol, vol.129, pp.1-13, 2009.

V. Le-moigne, R. Cariolet, V. Beven, A. Keranflec'h, A. Jestin et al., Electroporation improves the immune response induced by a DNA vaccine against pseudorabies virus glycoprotein B in pigs, Res. Vet. Sci, vol.93, pp.1032-1035, 2012.

V. Gerdts, A. Jons, B. Makoschey, N. Visser, and T. C. Mettenleiter, Protection of pigs against Aujeszky's disease by DNA vaccination, J Gen Virol, vol.78, pp.2139-2146, 1997.

D. Dory, M. Remond, V. Beven, R. Cariolet, S. Zientara et al., Foot-and-mouth disease virus neutralizing antibodies production induced by pcDNA3 and Sindbis virus based plasmid encoding FMDV P1-2A3C3D in swine, Antivir. Res, vol.83, pp.45-52, 2009.
URL : https://hal.archives-ouvertes.fr/hal-00404759

Y. Li, C. M. Stirling, M. S. Denyer, P. Hamblin, G. Hutchings et al., Dramatic improvement in FMD DNA vaccine efficacy and cross-serotype antibody induction in pigs following a protein boost, Vaccine, vol.26, pp.2647-2656, 2008.

A. Burgara-estrella, I. Diaz, I. M. Rodriguez-gomez, S. E. Essler, J. Hernandez et al., Predicted peptides from non-structural proteins of porcine reproductive and respiratory syndrome virus are able to induce IFN-gamma and IL-10, Viruses, vol.5, pp.663-677, 2013.

S. B. Morgan, S. P. Graham, F. J. Salguero, P. J. Sanchez-cordon, H. Mokhtar et al., Increased pathogenicity of European porcine reproductive and respiratory syndrome virus is associated with enhanced adaptive responses and viral clearance, Vet. Microbiol, vol.163, pp.13-22, 2013.

J. Lutz, S. Lazzaro, M. Habbeddine, K. E. Schmidt, P. Baumhof et al., Unmodified mRNA in LNPs constitutes a competitive technology for prophylactic vaccines, NPJ Vaccines, vol.2, p.29, 2017.

N. Pardi, M. J. Hogan, R. S. Pelc, H. Muramatsu, H. Andersen et al., Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination, Nature, vol.543, pp.248-251, 2017.

D. K. Edwards, E. Jasny, H. Yoon, N. Horscroft, B. Schanen et al., Adjuvant effects of a sequence-engineered mRNA vaccine: translational profiling demonstrates similar human and murine innate response, J. Transl. Med, vol.15, p.1, 2017.

C. Bernelin-cottet, Journal of Controlled Release, vol.308, pp.14-28, 2019.