Skip to Main content Skip to Navigation
Journal articles

Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato

Abstract : Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones.
Document type :
Journal articles
Complete list of metadata

Cited literature [67 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02622631
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Tuesday, May 26, 2020 - 6:15:11 AM
Last modification on : Wednesday, November 25, 2020 - 3:00:03 PM

File

Nahirnak-PLOS ONE-2019_1.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Vanesa Nahirñak, Máximo Rivarola, Natalia Inés Almasia, María Pilar Barrios Barón, Horacio Esteban Hopp, et al.. Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato. PLoS ONE, Public Library of Science, 2019, 14 (3), ⟨10.1371/journal.pone.0214165⟩. ⟨hal-02622631⟩

Share

Metrics

Record views

1159

Files downloads

138