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A standardized biohydrogen potential protocol: An international round robin test approach

Julian Carrillo-Reyes 1 Aída Tapia-Rodríguez 2 German Buitron 1 Iván Moreno-Andrade 1 Rodolfo Palomo-Briones 2 Elías Razo-Flores 2 Oscar Aguilar Juárez 3 Jorge Arreola-Vargas 4 Nicolas Bernet 5 Adriana Ferreira Maluf Braga 6 Lucia Braga 7 Elena Castelló 7 Lucille Chatellard 5 Claudia Etchebehere 8 Laura Fuentes 8 Elizabeth León-Becerril 3 Hugo Oscar Mendez-Acosta 9 Gonzalo Ruiz-Filippi 10 Estela Tapia-Venegas 10 Eric Trably 5 Jorge Wenzel 8 Marcelo Zaiat 6
1 UNAM - Universidad Nacional Autónoma de México
2 División de Ciencias Ambientales
3 CIATEJ - Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco
4 UTJ - Universidad Tecnologica de Jalisco
5 LBE - Laboratoire de Biotechnologie de l'Environnement [Narbonne]
6 USP - Universidade de São Paulo
7 Universidad de la República Uruguay
8 IIBCE - Instituto de Investigaciones Biológicas Clemente Estable
9 Departamento de Ingeniería Química [Guadalajara]
10 Escuela de Ingenieria Bioquimica
Abstract : Hydrogen production by dark fermentation is an emerging technology of increasing interest due to its renewable feature. Recent scientific advances have well investigated the operational conditions to produce hydrogen through the valorization of several wastes or wastewaters. However, the development of standardized protocols to accurately assess the biohydrogen potential (BHP) is of crucial importance. This work is the first interlaboratory and international effort to validate a protocol estimating hydrogen potential using batch tests, using glucose as individual model substrate. The repeatability of the hydrogen potential (HP) increased with variations of the proposed protocol: reducing substrate concentration, increasing the buffer capacity, and using an automatic device. The interlaboratory variation of the HP was reduced from 32 to 12%, demonstrating the reproducibility and robustness of the proposed protocol. Recommendations to run BHP tests were formulated in terms of i) repeatability and reproducibility of results, ii) criteria for results validation and acceptance, iii) workload of the proposed protocols.
Keywords : Batch protocol Dark fermentation Glucose Heat-treated inoculum
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Journal articles
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https://hal.inrae.fr/hal-02623601
Contributor : Migration Prodinra <>
Submitted on : Tuesday, May 26, 2020 - 8:41:23 AM
Last modification on : Friday, February 5, 2021 - 3:50:04 AM

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Julian Carrillo-Reyes, Aída Tapia-Rodríguez, German Buitron, Iván Moreno-Andrade, Rodolfo Palomo-Briones, et al.. A standardized biohydrogen potential protocol: An international round robin test approach. International Journal of Hydrogen Energy, Elsevier, 2019, 44 (48), pp.26237-26247. ⟨10.1016/j.ijhydene.2019.08.124⟩. ⟨hal-02623601⟩

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