Rapid sample preparation for ganglioside analysis by liquid chromatography mass spectrometry - Archive ouverte HAL Access content directly
Journal Articles Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences Year : 2020

Rapid sample preparation for ganglioside analysis by liquid chromatography mass spectrometry

(1, 2) , (1, 2) , (1, 2) , (1, 2) , (1, 2)
1
2

Abstract

Gangliosides (GG) are glycosphingolipids, composed of a ceramide moiety (fatty acid and long chain base) linked to an oligosaccharide chain containing one (or more) molecule of sialic acid. After lipid extraction from biological matrices, quantification of GG by liquid chromatography coupled to electrospray ionization mass spectrometry (LC-ESI/MS) can be impacted by ion suppression effects due to co-elution with more abundant lipids in the matrix. In this study, a simple, rapid and efficient method to purify GG from biological samples by Phree columns is proposed. This approach proved to be useful in eliminating phospholipids (PL) from the matrix and thus increasing the signal of GG classes and molecular species in rat brain samples during LC-ESI/MS analysis.
Fichier principal
Vignette du fichier
S1570023219310153.pdf (466.74 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-02625080 , version 1 (21-07-2022)

Licence

Attribution - NonCommercial - CC BY 4.0

Identifiers

Cite

Spiro Khoury, Elodie A.Y. Masson, Estelle Sibille, Stéphanie Cabaret, Olivier Berdeaux. Rapid sample preparation for ganglioside analysis by liquid chromatography mass spectrometry. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, 2020, 1137, pp.121956. ⟨10.1016/j.jchromb.2019.121956⟩. ⟨hal-02625080⟩
28 View
13 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More