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 Rapid sample preparation for ganglioside analysis by liquid chromatography mass spectrometry Spiro Khoury 1163341-0 Elodie A.Y. Masson b348ddf64afb2bfc56084c8abca5818d inrae.fr elodie-masson 735916 32180-735916 https://orcid.org/0000-0001-6312-0913 https://www.idref.fr/095193960 Estelle Sibille fa08fac1e0d415b9a3c446e8f2181668 inra.fr 1136712 933048-1136712 Stéphanie Cabaret 653012-0 Olivier Berdeaux 09655e7702105849d6344200f0853b91 inrae.fr olivier-berdeaux 1174289 232693-1174289 https://orcid.org/0000-0003-3675-0324 Accord Elsevier CCSD 3cc0cc7b8e3329bfb7423fa2578d788e ccsd.cnrs.fr 2022-07-21 09:55:50 2025-03-31 08:12:06 2022-07-21 09:55:53 2020-01-15 2020-12-28 0 inra_etatDocument_local_0 inra_publicVise_local_SC 1324-Centre des Sciences du Goût et de l'Alimentation (CSGA) 2017-07-01 contributor Accord Elsevier CCSD 3cc0cc7b8e3329bfb7423fa2578d788e ccsd.cnrs.fr CCSD hal-02625080 https://hal.inrae.fr/hal-02625080 khoury:hal-02625080 <i>Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences</i>, 2020, 1137, pp.121956. <a target="_blank" href="https://dx.doi.org/10.1016/j.jchromb.2019.121956">&#x27E8;10.1016/j.jchromb.2019.121956&#x27E9;</a> Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, 2020, 1137, pp.121956. &#x27E8;10.1016/j.jchromb.2019.121956&#x27E9; CC BY-NC 4.0 - Attribution - Non-commercial use Université Bourgogne Europe CNRS - Centre national de la recherche scientifique Centre des Sciences du Goût et de l'Alimentation Archive ouverte en agrobiosciences Institut National de Recherche en Agriculture, Alimentation et Environnement Import Elsevier Département Alimentation Humaine Institut Agro Indexation en cours. International No Yes Rapid sample preparation for ganglioside analysis by liquid chromatography mass spectrometry Spiro Khoury 1163341-0 Elodie A.Y. Masson b348ddf64afb2bfc56084c8abca5818d inrae.fr elodie-masson 735916 32180-735916 https://orcid.org/0000-0001-6312-0913 https://www.idref.fr/095193960 Estelle Sibille fa08fac1e0d415b9a3c446e8f2181668 inra.fr 1136712 933048-1136712 Stéphanie Cabaret 653012-0 Olivier Berdeaux 09655e7702105849d6344200f0853b91 inrae.fr olivier-berdeaux 1174289 232693-1174289 https://orcid.org/0000-0003-3675-0324 10993 1570-0232 1873-376X Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences Elsevier 1137 121956 2020-01-15 2019-12-23 10.1016/j.jchromb.2019.121956 S1570-0232(19)31015-3 491101 31891858 000509616900015 https://www.sciencedirect.com/journal/journal-of-chromatography-b English ion suppression quantitative analysis liquid chromatography-mass spectrometry purification lipids gangliosides Chemical Sciences/Analytical chemistry analyse quantitative chromatographie en phase liquide purification spectrométrie de masse absorption d'ion ganglioside lipide Journal articles Journal articles Journal articles

Gangliosides (GG) are glycosphingolipids, composed of a ceramide moiety (fatty acid and long chain base) linked to an oligosaccharide chain containing one (or more) molecule of sialic acid. After lipid extraction from biological matrices, quantification of GG by liquid chromatography coupled to electrospray ionization mass spectrometry (LC-ESI/MS) can be impacted by ion suppression effects due to co-elution with more abundant lipids in the matrix. In this study, a simple, rapid and efficient method to purify GG from biological samples by Phree columns is proposed. This approach proved to be useful in eliminating phospholipids (PL) from the matrix and thus increasing the signal of GG classes and molecular species in rat brain samples during LC-ESI/MS analysis.

 159748593 201019359D Centre des Sciences du Goût et de l'Alimentation [Dijon] CSGA 2020-01-01 2021-12-31
Université de Bourgogne - Bât. CSG - 9E Bd Jeanne d'Arc - 21000 Dijon
http://www2.dijon.inra.fr/csga/ 200716271 0000 0004 4910 6615 https://ror.org/02dn7x778 Université Bourgogne Franche-Comté [COMUE] UBFC 2015-04-01 2024-12-31
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http://www.ubfc.fr 02819005X 0000000122989313 https://ror.org/03k1bsr36 Université de Bourgogne UB 2024-12-31
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https://www.cnrs.fr/ https://ror.org/003vg9w96 Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement INRAE 2020-01-01