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Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor

Abstract : The hydrogen (H-2) production efficiency in dark fermentation systems is strongly dependent on the occurrence of metabolic pathways derived from the selection of microbial species that either consume molecular H-2 or outcompete hydrogenogenic bacteria for the organic substrate. In this study, the effect of organic loading rate (OLR) on the H-2 production performance, the metabolic pathways, and the microbial community composition in a continuous system was evaluated. Two bacterial genera, Clostridium and Streptococcus, were dominant in the microbial community depending on the OLR applied. At low OLR (14.7-44.1 g(Lactose)/L-d), Clostridium sp. was dominant and directed the system towards the acetate-butyrate fermentation pathway, with a maximum H-2 yield of 2.14 mol(H2)/mol(Hexose) obtained at 29.4 g(Lactose)/L-d. Under such conditions, the volumetric hydrogen production rate (VHPR) was between 3.2 and 11.6 L-H2/L-d. In contrast, relatively high OLR (58.8 and 88.2 g(Lactose)/L-d) favored the dominance of Streptococcus sp. as co-dominant microorganism leading to lactate production. Under these conditions, the formate production was also stimulated serving as a strategy to dispose the surplus of reduced molecules (e.g., NADH(2) (+)), which theoretically consumed up to 5.72 L-H2/L-d. In such scenario, the VHPR was enhanced (13.7-14.5 L-H2/L-d) but the H-2 yield dropped to a minimum of 0.74 mol(H2)/mol(Hexose) at OLR = 58.8 g(Lactose)/L-d. Overall, this research brings clear evidence of the intrinsic occurrence of metabolic pathways detrimental for biohydrogen production, i.e., lactic acid fermentation and formate production, suggesting the use of low OLR as a strategy to control them.
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https://hal.inrae.fr/hal-02625208
Contributor : Migration Prodinra <>
Submitted on : Tuesday, May 26, 2020 - 1:46:17 PM
Last modification on : Thursday, November 5, 2020 - 9:07:42 AM

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R. Palomo-Briones, Eric Trably, N. E. López-Lozano, L. B. Celis, H. O. Méndez-Acosta, et al.. Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor. Applied Microbiology and Biotechnology, Springer Verlag, 2018, 102 (5), pp.2465-2475. ⟨10.1007/s00253-018-8737-7⟩. ⟨hal-02625208⟩

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