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1H-NMR metabolomics response to a realistic diet contamination with the mycotoxin deoxynivalenol: Effect of probiotics supplementation

Abstract : Low-level contamination of food and feed by mycotoxin deoxynivalenol (DON) is unavoidable. We investigated the effects of subclinical challenge with DON, and dietary supplementation with probiotic yeast Saccharomyces cerevisiae boulardii I1079 as preventive strategy. Thirty-six piglets were randomly assigned to four different diets: control diet, diet contaminated with DON (3 mg/kg), diet supplemented with yeast (4x10 9 CFU/kg), or DON-contaminated diet supplemented with yeast, for four weeks. Plasma samples were collected for biochemistry, and tissue samples for histology. 1 H-NMR untargeted metabolomics of plasma and liver were also explored. DON induced no significant modification of biochemistry parameters. However, higher lesional scores were observed and metabolomics highlighted alteration of the metabolism of amino acids and 2-oxocarboxylic acids. Administration of yeast impacted aminoacyl-tRNA synthesis and metabolism of amino acids and glycerophospholipids. Yeast supplementation to DON-exposed piglets prevented histological alterations, while partial least square discriminant analysis underlined similarity of their plasma metabolic profile to control group. In contrast to plasma, the effect on liver metabolome remained marginal, indicating that the toxicity of the mycotoxin was not abolished. These data indicate that 1 H-NMR metabolomics profile is a good biomarker for subclinical exposure to DON, and supplementation with S. cerevisiae boulardii increases piglet resilience to this mycotoxin.
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Submitted on : Wednesday, September 1, 2021 - 3:45:06 PM
Last modification on : Friday, September 10, 2021 - 3:02:03 AM

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Imourana Alassane-Kpembi, Cecile Canlet, Marie Tremblay-Franco, Fabien Jourdan, Maxime Chalzaviel, et al.. 1H-NMR metabolomics response to a realistic diet contamination with the mycotoxin deoxynivalenol: Effect of probiotics supplementation. Food and Chemical Toxicology, Elsevier, 2020, 138, pp.111222. ⟨10.1016/j.fct.2020.111222⟩. ⟨hal-03331253⟩

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