Gut Microbiota Interacts with Markers of Adipose Tissue Browning, Insulin Action and Plasma Acetate in Morbid Obesity - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Molecular Nutrition and Food Research Year : 2018

Gut Microbiota Interacts with Markers of Adipose Tissue Browning, Insulin Action and Plasma Acetate in Morbid Obesity

Matteo Sérino
Marc-Emmanuel Dumas

Abstract

Scope: To examine the potential relationship among gene expression markers of adipose tissue browning, gut microbiota, and insulin sensitivity in humans. Methods and results: Gut microbiota composition and gene markers of browning are analyzed in subcutaneous (SAT) and visceral (VAT) adipose tissue from morbidly obese subjects (n = 34). Plasma acetate is measured through H-1 NMR and insulin sensitivity using euglycemic hyperinsulinemic clamp. Subjects with insulin resistance show an increase in the relative abundance (RA) of the phyla Bacteroidetes and Proteobacteria while RA of Firmicutes is decreased. In all subjects, Firmicutes RA is negatively correlated with HbA(1c) and fasting triglycerides, whereas Proteobacteria RA was negatively correlated with insulin sensitivity. Firmicutes RA is positively associated with markers of brown adipocytes (PRDM16, UCP1, and DIO2) in SAT, but not in VAT. Multivariate regression analysis indicates that Firmicutes RA contributes significantly to SAT PRDM16, UCP1, and DIO2 mRNA variance after controlling for age, BMI, HbA(1c), or insulin sensitivity. Interestingly, Firmicutes RA, specifically those bacteria belonging to the Ruminococcaceae family, is positively associated with plasma acetate levels, which are also linked to SAT PRDM16 mRNA and insulin sensitivity. Conclusion: Gut microbiota composition is linked to adipose tissue browning and insulin action in morbidly obese subjects, possibly through circulating acetate.
Fichier principal
Vignette du fichier
last submitted version.pdf (784.61 Ko) Télécharger le fichier

Dates and versions

hal-02627928 , version 1 (01-04-2024)

Identifiers

Cite

José Maria Moreno-Navarrete, Matteo Sérino, Vincent Blasco-Baque, Vincent Azalbert, Richard H. Barton, et al.. Gut Microbiota Interacts with Markers of Adipose Tissue Browning, Insulin Action and Plasma Acetate in Morbid Obesity. Molecular Nutrition and Food Research, 2018, 62 (3), ⟨10.1002/mnfr.201700721⟩. ⟨hal-02627928⟩
49 View
15 Download

Altmetric

Share

More