Novel fibers containing different ratios of PVA and D-limonene were fabricated using electrospinning for antibacterial active packaging applications. The PVA/D-limonene fibers were thoroughly characterized using a scanning electron microscope, fourier-transform infrared spectrometry, thermal gravimetry, differential scanning calorimetry, tensile tests, and oxygen permeability tests. The results of these analyses showed that the highest tensile strength and elongation at break values of 3.87 [u]+[/u] 0.25 MPa and 55.62 [u]+[/u] 2.93%, respectively, were achieved for a PVA/D-limonene ratio of 7:3 (v/v) and an ultrasonication time of 15 min during processing. This material also showed the lowest oxygen permeation and the best degradability and bacteriostatic properties of all samples.