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Article Dans Une Revue Nature Communications Année : 2018

A mosaic monoploid reference sequence for the highly complex genome of sugarcane

Rudie Antonise
  • Fonction : Auteur

Résumé

Sugarcane (Saccharum spp.) is a major crop for sugar and bioenergy production. Its highly polyploid, aneuploid, heterozygous, and interspecific genome poses major challenges for producing a reference sequence. We exploited colinearity with sorghum to produce a BAC-based monoploid genome sequence of sugarcane. A minimum tiling path of 4660 sugarcane BAC that best covers the gene-rich part of the sorghum genome was selected based on whole-genome profiling, sequenced, and assembled in a 382-Mb single tiling path of a high-quality sequence. A total of 25,316 protein-coding gene models are predicted, 17% of which display no colinearity with their sorghum orthologs. We show that the two species, S. officinarum and S. spontaneum, involved in modern cultivars differ by their transposable elements and by a few large chromosomal rearrangements, explaining their distinct genome size and distinct basic chromosome numbers while also suggesting that polyploidization arose in both lineages after their divergence.
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hal-02628799 , version 1 (26-05-2020)

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Olivier Garsmeur, Gaëtan Droc, Rudie Antonise, Jane Grimwood, Bernard Potier, et al.. A mosaic monoploid reference sequence for the highly complex genome of sugarcane. Nature Communications, 2018, 9 (1), 10 p. ⟨10.1038/s41467-018-05051-5⟩. ⟨hal-02628799⟩
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