Pseudomonas savastanoi pv. savastanoi, the causal agent of olive knot disease, often switches from a saprophytic to a parasitic lifestyle only following natural or man-made activities, that cause lesions on plant tissues. We investigated the possible presence of this pathogen on the phylloplane of 28 Italian olive cultivars, recently introduced to Nepal. Although a consistent number of bacterial species were found in association with leaf, there was no presence of olive knot pathogen across the study area. The results suggest that the introduction of a new plant species in a given area does not necessarily introduce the pathogen when the propagation materials are rigorously supervised for pathogen exclusion.