Skip to Main content Skip to Navigation
Journal articles

Food deprivation affects the miRNome in the lactating goat mammary gland

Abstract : Nutrition affects milk composition thus influencing its nutritional properties. Nutrition also modifies the expression of mammary genes, whose regulation is not fully understood. MicroRNAs (miRNA) are small non coding RNA which are important post-transcriptional regulators of gene expression by targeting messenger RNAs. Our goal was to characterize miRNA whose expression is regulated by nutrition in the lactating goat mammary gland, which may provide clues to deciphering regulations of the biosynthesis and secretion of milk components. Using high-throughput sequencing technology, miRNomes of the lactating mammary gland were established from lactating goats fed ad libitum or deprived of food for 48h affecting milk production and composition. High throughput miRNA sequencing revealed 30 miRNA with an expression potentially modulated by food deprivation; 16 were down-regulated and 14 were up-regulated. Diana-microT predictive tools suggested a potential role for several nutriregulated miRNA in lipid metabolism. Among the putative targets, 19 were previously identified as differently expressed genes (DEG). The functions of these 19 DEG revealed, notably, their involvement in tissue remodelling. In conclusion, this study offers the first evidence of nutriregulated miRNA in the ruminant mammary gland. Characterization of these 30 miRNA could contribute to a clearer understanding of gene regulation in the mammary gland in response to nutrition.
Complete list of metadata

Cited literature [69 references]  Display  Hide  Download
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Wednesday, May 27, 2020 - 6:54:08 AM
Last modification on : Friday, November 5, 2021 - 2:50:22 PM


Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution 4.0 International License



Lenha Mobuchon, Sylvain Marthey, Sandrine Le Guillou, Denis Laloë, Fabienne Le Provost, et al.. Food deprivation affects the miRNome in the lactating goat mammary gland. PLoS ONE, Public Library of Science, 2015, 10 (10), 20 p. ⟨10.1371/journal.pone.0140111⟩. ⟨hal-02630964⟩



Record views


Files downloads