, Eraso Centelles (Director del Servicio de Conservación de la Biodiversidad), Mr. Martínez García (Técnico de la Sección de Hábitats) and Mr. Larumbe Arricibita (Jefe de la Sección de Hábitats)). Year, France was carried out with collecting permit (Arrêté n°2008.38.4) issued by Préfecture des Hautes Alpes. Fieldwork in Spain was specifically approved by the following institutions: Generalitat de Catalunya (Ref.: 0152S), vol.30297, 2007.

K. W. Matsubayashi, S. Kahono, and H. Katakura, Divergent host plant specialization as the critical driving force in speciation between populations of a phytophagous ladybird beetle, J Evol Biol, vol.24, pp.1421-1453, 2011.

T. Powell, A. Forbes, G. Hood, and J. Feder, Ecological adaptation and reproductive isolation in sympatry: genetic and phenotypic evidence for native host races of Rhagoletis pomonella, Mol Ecol, vol.23, pp.688-704, 2014.

T. Nyman, V. Vikberg, D. Smith, and J. Boeve, How common is ecological speciation in plant-feeding insects? A 'Higher' Nematinae perspective, BMC Evol Biol, vol.10, p.266, 2010.

J. Brown, J. Leebensmack, J. Thompson, O. Pellmyr, and R. Harrison, Phylogeography and host association in a pollinating seed parasite Greya politella (Lepidoptera: Prodoxidae), Mol Ecol, vol.6, pp.215-239, 1997.

P. Mardulyn, N. Othmezouri, Y. Mikhailov, and J. Pasteels, Conflicting mitochondrial and nuclear phylogeographic signals and evolution of host-plant shifts in the boreo-montane leaf beetle Chrysomela lapponica, Mol Phylogenet Evol, vol.61, pp.686-96, 2011.

C. Darwell, K. Fox, and D. Althoff, The roles of geography and founder effects in promoting host-associated differentiation in the generalist bogus yucca moth Prodoxus decipiens, J Evol Biol, vol.27, pp.2706-2724, 2014.

F. Bensettiti and V. Gaudillat, Graellsia isabellae (Graëlls, 1849) L'Isabelle de France, le Papillon vitrail. Insectes, Lépidoptères, Saturniides, Connaissance et gestion des habitats et des espèces d'intérèt communautaire, Tome 7. Espèces animales, pp.277-286, 2000.

J. R. Verdú, C. Numa, and E. Galante, Atlas y libro rojo de los invertebrados amenazados de España (Especies Vulnerables), vol. I: Artrópodos. Madrid: Dirección General de Medio Natural y Política Forestal, 2011.

M. Moreno, R. , H. Alonso, and R. , Graellsia isabelae. Vida Silvestre, vol.12, pp.207-227, 1975.

R. Chefaoui and J. Lobo, Assessing the conservation status of an Iberian moth using pseudo-absences, J Wildl Manage, vol.71, pp.2507-2523, 2007.

A. Crespo, J. I. , J. Mendoza, S. , S. Fernández et al., Recopilación de la información biogeográfica, análisis de patrones ecológicos, conservación y mapa potencial de Graellsia isabelae (Graells, 1849) (Lepidoptera, Saturniidae) en la provincia de Cuenca, España Graellsia, vol.66, pp.9-20, 2010.

G. Bustillo, M. R. Fernández-rubio, and F. , Consideraciones sobre la planta nutricia de Graellsia isabelae, SHILAP Soc Hispano Luso Am Lepid, vol.7, pp.183-192, 1974.

S. Ibáñez-gázquez, N. Ariza, J. C. , Y. Ullastre, and J. , Graellsia isabelae (Graells, 1849), una nueva especie para la fauna lepidopterológica de Almería (España) (Lepidoptera: Saturniidae), SHILAP Soc Hispano Luso Am Lepid, vol.36, pp.427-457, 2008.

J. Fürstenberg-hägg, M. Zagrobelny, and S. Bak, Plant defense against insect herbivores, Int J Mol Med Sci, vol.14, pp.10242-97, 2013.

P. Quézel and F. Médail, Écologie et biogéographie des forêts du bassin méditerranéen. Collection Environnement, pp.187-200, 2003.

R. Cheddadi, G. Vendramin, T. Litt, L. Francois, M. Kageyama et al., Imprints of glacial refugia in the modern genetic diversity of Pinus sylvestris, Glob Ecol Biogeogr, vol.15, pp.271-82, 2006.
URL : https://hal.archives-ouvertes.fr/halsde-00303681

Z. Afzal-rafii and R. Dodd, Chloroplast DNA supports a hypothesis of glacial refugia over postglacial recolonization in disjunct populations of black pine (Pinus nigra) in Western Europe, Mol Ecol, vol.16, pp.723-759, 2007.

B. Garzón, M. , S. De-dios, R. , S. Ollero et al., Predictive modelling of tree species distributions on the Iberian Peninsula during the Last Glacial Maximum and Mid-Holocene, Ecography, vol.30, pp.120-154, 2007.

B. Garzón, M. , S. De-dios, R. , S. Ollero et al., The evolution of the Pinus sylvestris L. area in the Iberian Peninsula from the last glacial maximum to 2100 under climate change, Holocene, vol.18, pp.705-719, 2008.

M. Vila, N. Marí-mena, Y. S. Lopez-vaamonde, and C. , Characterization of ten polymorphic microsatellite markers for the protected Spanish moon moth Graellsia isabelae (Lepidoptera: Saturniidae), Conserv Genet, vol.11, pp.1151-1155, 2010.

A. Soto, J. Robledo-arnuncio, S. González-martínez, P. Smouse, and R. Alía, Climatic niche and neutral genetic diversity of the six Iberian pine species: a retrospective and prospective view, Mol Ecol, vol.19, pp.1396-409, 2010.

J. Millar, J. Mcelfresh, C. Romero, M. Vila, N. Marí-mena et al., Identification of the Sex pheromone of a protected species, the Spanish moon moth Graellsia isabellae, J Chem Ecol, vol.36, pp.923-955, 2010.

M. Vila, M. Auger-rozenberg, F. Goussard, and C. Lopez-vaamonde, Effect of nonlethal sampling on life-history traits of the protected moth Graellsia isabelae (Lepidoptera: Saturniidae), Ecol Entomol, vol.34, pp.356-62, 2009.

C. Simon, F. Frati, A. Beckenback, B. Crespi, H. Liu et al., Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers, Ann Entomol Soc Am, vol.87, pp.651-701, 1994.

M. Vila and M. Björklund, The utility of the neglected mitochondrial control region for evolutionary studies in Lepidoptera (Insecta), J Mol Evol, vol.58, pp.280-90, 2004.

P. Librado and J. Rozas, DnaSP v5: a software for comprehensive analysis of DNA polymorphism data, Bioinformatics, vol.25, pp.1451-1453, 2009.

J. Goudet, FSTAT (version 1.2): a computer program to calculate F-statistics, J Hered, vol.86, pp.485-491, 1995.

S. Kalinowski, HP-RARE 1.0: a computer program for performing rarefaction on measures of allelic richness, Mol Ecol Notes, vol.5, pp.187-196, 2005.

F. Rousset and . Genepop, 007: a complete re-implementation of the GENEPOP software for Windows and Linux, Mol Ecol Res, vol.8, pp.103-109, 2008.
URL : https://hal.archives-ouvertes.fr/halsde-00366752

L. Excoffier and H. Lischer, Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows, Mol Ecol Res, vol.10, pp.564-571, 2010.

I. Dupanloup, S. Schneider, and L. Excoffier, A simulated annealing approach to define the genetic structure of populations, Mol Ecol, vol.11, pp.2571-81, 2002.

J. Corander, P. Marttinen, J. Sirén, and J. Tang, Enhanced Bayesian modelling in BAPS software for learning genetic structures of populations, BMC Bioinformatics, vol.9, 2008.

L. Cheng, T. Connor, J. Sirén, D. Aanensen, and J. Corander, Hierarchical and spatially explicit clustering of DNA sequences with BAPS software, Mol Biol Evol, vol.30, pp.1224-1232, 2013.

J. Pritchard, M. Stephens, and P. Donnelly, Inference of population structure using multilocus genotype data, Genetics, vol.155, pp.945-59, 2000.

D. Falush, M. Stephens, and J. Pritchard, Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies, Genetics, vol.164, pp.1567-87, 2003.

G. Evanno, S. Regnaut, and J. Goudet, Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study, Mol Ecol, vol.14, pp.2611-2631, 2005.

M. Jakobsson and N. Rosenberg, CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure, Bioinformatics, vol.23, pp.1801-1807, 2007.

N. Rosenberg, DISTRUCT: a program for the graphical display of population structure, Mol Ecol Notes, vol.4, pp.137-145, 2004.

E. Minch, A. Ruiz-linares, D. Goldstein, M. Feldman, and L. Cavalli-sforza, Microsat: a computer program for calculating various statistics on microsatellite allele data. Standford: Standford University Medical Center, 1996.

J. Felsenstein and . Phylip, Phylogenetic Inference Package) 3.68. Distributed by the author, 2005.

M. Clement, D. Posada, and K. Crandall, TCS: a computer program to estimate gene genealogies, Mol Ecol, vol.9, pp.1657-1666, 2000.

F. Ronquist, M. Teslenko, P. Van-der-mark, D. Ayres, A. Darling et al., MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space, Syst Biol, vol.61, pp.539-581, 2012.

J. Huelsenbeck, B. Larget, and M. Alfaro, Bayesian phylogenetic model selection using reversible jump Markov chain Monte Carlo, Mol Biol Evol, vol.21, pp.1123-1156, 2004.

R. Kass and A. Raftery, Bayes factors, J Am Stat Assoc, vol.90, pp.773-95, 1995.

A. Papadopoulou, I. Anastasiou, and A. Vogler, Revisiting the insect mitochondrial molecular clock: the Mid-Aegean trench calibration, Mol Biol Evol, vol.27, pp.1659-72, 2010.

S. Schneider and L. Excoffier, Estimation of past demographic parameters from the distribution of pairwise differences when the mutation rates very among sites: application to human mitochondrial DNA, Genetics, vol.152, pp.1079-89, 1999.

A. Rogers and H. Harpending, Population growth makes waves in the distribution of pairwise genetic differences, Mol Biol Evol, vol.9, pp.552-69, 1992.

S. Ramos-onsins and J. Rozas, Statistical properties of new neutrality tests against population growth, Mol Biol Evol, vol.19, pp.2092-100, 2002.

A. Ramírez-soriano, S. Ramos-onsins, J. Rozas, F. Calafell, and A. Navarro, Statistical power analysis of neutrality tests under demographic expansions, contractions and bottlenecks with recombination, Genetics, vol.179, pp.555-67, 2008.

S. Piry, G. Luikart, and J. Cornuet, BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data, J Hered, vol.90, pp.502-505, 1999.

G. Luikart, F. Allendorf, J. Cornuet, and W. Sherwin, Distortion of allele frequency distributions provides a test for recent population bottlenecks, J Hered, vol.89, pp.238-285, 1998.

W. Sinclair, J. Morman, and R. Ennos, The postglacial history of Scots pine (Pinus sylvestris L.) in Western Europe: evidence from mitochondrial DNA variation, Mol Ecol, vol.8, pp.83-91, 1999.

N. Soranzo, A. R. Provan, J. Powell, and W. , Patterns of variation at a mitochondrial sequence-tagged-site locus provides new insights into the postglacial history of European Pinus sylvestris populations, Mol Ecol, vol.9, pp.1205-1216, 2000.

K. Naydenov, S. Senneville, J. Beaulieu, F. Tremblay, and J. Bousquet, Glacial vicariance in Eurasia: mitochondrial DNA evidence from Scots pine for a complex heritage involving genetically distinct refugia at mid-northern latitudes and in Asia Minor, BMC Evol Biol, vol.7, p.233, 2007.

T. Pyhäjärvi, M. Salmela, and O. Savolainen, Colonization routes of Pinus sylvestris inferred from distribution of mitochondrial DNA variation, Tree Genet Genomes, vol.4, pp.247-54, 2008.

J. Robledo-arnuncio, C. Collada, R. Alía, and L. Gil, Genetic structure of montane isolates of Pinus sylvestris L. in a Mediterranean refugial area, J Biogeogr, vol.32, pp.595-605, 2005.

W. Prus-glowacki, S. B. Bujas, E. , A. R. Marciniak, and A. , Genetic differentiation of autochthonous populations of Pinus sylvestris (Pinaceae) from the Iberian Peninsula, Plant Syst Evol, vol.239, pp.55-66, 2003.

J. Jaramillo-correa, D. Grivet, A. Terrab, Y. Kurt, A. De-lucas et al., The Strait of Gibraltar as a major biogeographic barrier in Mediterranean conifers: a comparative phylogeographic survey, Mol Ecol, vol.19, pp.5452-68, 2010.

A. Rubio-moraga, D. Candel-perez, M. Lucas-borja, P. Tiscar, B. Viñegla et al., Genetic diversity of Pinus nigra Arn. Populations in southern Spain and northern morocco revealed by intersimple sequence repeat profiles, Int J Mol Sci, vol.13, pp.5645-58, 2012.

T. Sasaki, T. Kubo, and H. Ishikawa, Interspecific transfer of Wolbachia between two lepidopteran insects expressing cytoplasmic incompatibility: a Wolbachia variant naturally infecting Cadra cautella causes male killing in Ephestia kuehniella, Genetics, vol.162, pp.1313-1322, 2002.

U. Kodandaramaiah, T. Simonsen, S. Bromilow, N. Wahlberg, and F. Sperling, Deceptive single-locus taxonomy and phylogeography: Wolbachiaassociated divergence in mitochondrial DNA is not reflected in morphology and nuclear markers in a butterfly species, Ecol Evol, vol.3, pp.5167-76, 2013.

G. Del-barrio, J. Auñón, F. , S. De-ron, and D. , GIS of forest tree species of Spain. Instituto Nacional de Investigación y Tecnología Agraria y Alimentación, 2015.

V. Dvornyk, A. Sirvio, M. Mikkonen, and O. Savolainen, Low nucleotide diversity at the pal1 locus in the widely distributed Pinus sylvestris, Mol Biol Evol, vol.19, pp.179-88, 2002.

T. Pyhäjärvi, M. García-gil, T. Knurr, M. Mikkonen, W. Wachowiak et al., Demographic history has influenced nucleotide diversity in European Pinus sylvestris populations, Genetics, vol.177, pp.1713-1737, 2007.

J. Cipriano, A. Carvalho, C. Fernandes, M. Gaspar, J. Pires et al., Evaluation of genetic diversity of Portuguese Pinus sylvestris L. populations based on molecular data and inferences about the future use of this germplasm, J Genet, vol.92, pp.41-49, 2013.

H. Romo, E. García-barros, J. Martín, J. Ylla, M. López et al., Bases ecológicas preliminares para la conservación de las especies de interés comunitario en España: Invertebrados. Madrid: Ministerio de Agricultura, Alimentación y Medio Ambiente, pp.1-53, 2012.

J. Rousselet, R. Zhao, D. Argal, M. Simonato, A. Battisti et al., The role of topography in structuring the demographic history of the pine processionary moth, Thaumetopoea pityocampa (Lepidoptera: Notodontidae), J Biogeogr, vol.37, pp.1478-90, 2010.

E. Recuero and M. Garcia-parís, Evolutionary history of Lissotriton helveticus: Multilocus assessment of ancestral vs. recent colonization of the Iberian Peninsula, Mol Phylogenet Evol, vol.60, pp.170-82, 2011.

K. Mccracken and M. Sorenson, Is homoplasy or lineage sorting the source of incongruent mtDNA and nuclear gene trees in the stiff-tailed ducks (Nomonyx-Oxyura)?, Syst Biol, vol.54, pp.35-55, 2005.

B. Egger, S. Koblmüller, C. Sturmbauer, and K. M. Sefc, Nuclear and mitochondrial data reveal different evolutionary processes in the Lake Tanganyika cichlid genus Tropheus, BMC Evol Biol, vol.7, p.137, 2007.

G. Piganeau, M. Gardner, and A. Eyre-walker, A broad survey of recombination in animal mitochondria, Mol Biol Evol, vol.21, pp.2319-2344, 2004.

A. Tsaousis, D. Martin, E. Ladoukakis, D. Posada, and E. Zouros, Widespread recombination in published animal mtDNA sequences, Mol Biol Evol, vol.22, pp.925-958, 2005.

D. Gotzek, J. Clarke, and D. Shoemaker, Mitochondrial genome evolution in fire ants (Hymenoptera: Formicidae), BMC Evol Biol, vol.10, p.300, 2010.

K. Arunkumar, M. Metta, and J. Nagaraju, Molecular phylogeny of silkmoths reveals the origin of domesticated silkmoth, Bombyx mori from Chinese Bombyx mandarina and paternal inheritance of Antheraea proylei mitochondrial DNA, Mol Phylogenet Evol, vol.40, pp.419-446, 2006.

M. Dubreuil, M. Riba, and M. Mayol, Genetic structure and diversity in Ramonda myconi (Gesneriaceae): effects of historical climate change on a preglacial relict species, Am J Bot, vol.95, pp.577-87, 2008.

J. Templado, J. Álvarez, and E. Ortiz, Observaciones biológicas y citogenéticas sobre Graellsia isabelae (Graells 1849) (Lepidoptera: Saturniidae), Eos, vol.49, pp.285-92, 1975.

E. H. Fernández-vidal, Comentarios acerca de la distribución geográfica francesa y notas taxonómicas sobre Graellsia isabelae (Graells, 1849) (Lepidoptera: Saturniidae), SHILAP Soc Hispano Luso Am Lepid, vol.20, pp.29-49, 1992.

A. Cassel-lundhagen, C. Ronnås, A. Battisti, J. Wallén, and S. Larsson, Steppingstone expansion and habitat loss explain a peculiar genetic structure and distribution of a forest insect, Mol Ecol, vol.22, pp.3362-75, 2013.

P. Roiron, L. Chabal, I. Figueiral, J. Terral, and A. A. Ali, Palaeobiogeography of Pinus nigra Arn. subsp salzmannii (Dunal) Franco in the north-western Mediterranean Basin: a review based on macroremains, Rev Palaeobot Palynol, vol.194, pp.1-11, 2013.
URL : https://hal.archives-ouvertes.fr/hal-02321372

M. Förschler, J. Senar, A. Borras, J. Cabrera, and M. Björklund, Gene flow and range expansion in a mountain-dwelling passerine with a fragmented distribution, Biol J Linn Soc Lond, vol.103, pp.707-728, 2011.

L. Masutti, A. Battisti, and M. Faccoli, Insect fauna of the Pinus nigra group in Italy, Entomological research in Mediterranean forest ecosystems, pp.79-87, 2005.

D. Zovi, M. Stastny, A. Battisti, and S. Larsson, Ecological costs on local adaptation of an insect herbivore imposed by host plants and enemies, Ecology, vol.89, pp.1388-98, 2008.

M. Paiva, E. Mateus, M. Santos, and M. Branco, Pine volatiles mediate host selection for oviposition by Thaumetopoea pityocampa

, J Appl Entomol, vol.135, pp.195-203, 2011.

J. A. Hódar, A. Cassel-lundhagen, A. Battisti, and S. Larsson, A little further south: host range and genetics of the Northern pine processionary moth, Thaumetopoea pinivora (Lepidoptera: Notodontidae) at the southern edge of its distribution, Eur J Entomol, vol.113, pp.200-206, 2016.

V. Dinca, L. Dapporto, and R. Vila, A combined genetic-morphometric analysis unravels the complex biogeographical history of Polyommatus icarus and Polyommatus celina Common Blue butterflies, Mol Ecol, vol.20, pp.3921-3956, 2011.

A. Miraldo, G. Hewitt, P. O. Emerson, and B. , Phylogeography and demographic history of Lacerta lepida in the Iberian Peninsula: multiple refugia, range expansions and secondary contact zones, BMC Evol Biol, vol.11, p.170, 2011.

G. Bucci, S. González-martínez, L. Provost, G. Plomion, C. Ribeiro et al., Range-wide phylogeography and gene zones in Pinus pinaster Ait. revealed by chloroplast microsatellite markers, Mol Ecol, vol.16, pp.2137-53, 2007.

J. Ylla-i-ullastre, Història natural del lepidòpter Graellsia isabelae (Graells, 1849). Barcelona: Institut d'Estudis Catalans, 1997.

M. Simonato, Z. Mendel, C. Kerdelhué, J. Rousselet, E. Magnoux et al., Phylogeography of the pine processionary moth Thaumetopoea wilkinsoni in the Near East, Mol Ecol, vol.16, pp.2273-83, 2007.

R. Burton and F. Barreto, A disproportionate role for mtDNA in Dobzhansky-Muller incompatibilities?, Mol Ecol, vol.21, pp.4942-57, 2012.

J. N. Wolff, E. D. Ladoukakis, J. A. Enríquez, and D. K. Dowling, Mitonuclear interactions: evolutionary consequences over multiple biological scales, Phil Trans R Soc B, vol.369, 2014.

B. Milá, Y. Surget-groba, B. Heulin, A. Gosá, and P. Fitze, Multilocus phylogeography of the common lizard Zootoca vivipara at the Ibero-Pyrenean suture zone reveals lowland barriers and high-elevation introgression, BMC Evol Biol, vol.13, p.192, 2013.

G. Jalut, J. Marti, M. Fontugne, G. Delibrias, J. Vilaplana et al., Glacial to interglacial vegetation changes in the northern and southern Pyrénées: Deglaciation, vegetation cover and chronology, Quat Sci Rev, vol.11, pp.449-80, 1992.

P. Van-dijk and T. Bakx-schotman, Chloroplast DNA phylogeography and cytotype geography in autopolyploid Plantago media, Mol Ecol, vol.6, pp.345-52, 1997.

O. Charrier, P. Dupont, A. Pornon, and N. Escaravage, Microsatellite marker analysis reveals the complex phylogeographic history of Rhododendron ferrugineum (ericaceae) in the Pyrenees, PLoS One, vol.9, p.92976, 2014.

N. Marí-mena, C. Lopez-vaamonde, H. Naveira, M. A. Auger-rozenberg, and M. Vila, Microsatellite datafile GENEPOP format.txt. Data from: Phylogeography of the Spanish Moon Moth Graellsia isabellae

, , p.15, 2016.

N. Marí-mena, C. Lopez-vaamonde, H. Naveira, M. A. Auger-rozenberg, and M. Vila, Mitochondrial haplotype dataset. Data from: Phylogeography of the Spanish Moon Moth Graellsia isabellae (Lepidoptera, Saturniidae). figshare, 2016.

, , p.15, 2016.

O. Collectif, Contribution à la connaissance de Graellsia isabelae galliaegloria Oberthur (Lepidoptera, Attacidae) connu uniquement en France. Rapport d'études de l'OPIE, vol.3, pp.1-36, 1998.