V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Nature Plants Year : 2015

V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis

Samantha S. Vernhettes
Staffan Persson
  • Function : Author
  • PersonId : 969498
Eugenia Russinova

Abstract

In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants.
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Dates and versions

hal-02635674 , version 1 (29-02-2024)

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Yu Luo, Stefan Scholl, Anett Doering, Yi Zhang, Niloufer G. Irani, et al.. V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis. Nature Plants, 2015, 1 (7), ⟨10.1038/NPLANTS.2015.94⟩. ⟨hal-02635674⟩
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