Physiological responses of the hybrid larch (Larix x eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Environmental Science and Pollution Research Année : 2016

Physiological responses of the hybrid larch (Larix x eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets

Résumé

Phytoextraction of Cd is a growing biotechnology although we currently know few Cd-hyperaccumulators, i.e. plant species able to accumulate at least 0.1 mg Cd g-1 dry weight in aerial organs. Owing their deep root system and high biomass, trees are more and more preferred to herbaceous species for phytoextraction. Assuming that conifers could be relevant models under cold climates, we investigated cadmium tolerance of the hybrid larch Larix × eurolepis Henry (L. decidua × L. kaempferi) and the efficiency of this species to store this metal. In vitro grown larches were chosen in order to reduce time of exposure and to more rapidly evaluate their potential efficiency to accumulate Cd. One-month-old plantlets were exposed for 2 and 4 weeks to 250 and 500 µM Cd. Results showed that they tolerated a four-week exposure to 250 µM Cd whereas the content of photosynthetic pigment strongly dropped in plantlets growing in presence of 500 µM Cd. In presence of 250 µM Cd, shoot growth slightly decreased but photosynthetic pigment and total soluble carbohydrate contents were not modified and no lipid peroxidation was detected. In addition, these plantlets accumulated proline, particularly in shoots (2 - 3 times more than control). In roots, Cd concentration in the intracellular fraction was always higher than in the cell wall fraction contrary to shoots where Cd concentration in the cell wall fraction increased with time and Cd concentration in the medium. In shoots, Cd concentration was lower than in roots with a ratio of 0.2 after 4 weeks of exposure but stayed around 0.2 mg g-1 dry weight, thus a value higher than the threshold requested for Cd-hyperaccumulators. Hybrid larch would thus be a relevant candidate for field test of Cd-phytoextraction.
Fichier principal
Vignette du fichier
2016_Bonet_Environmental Science and Pollution Research (Postprint)_1.pdf (3.91 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02636839 , version 1 (27-05-2020)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Amandine Bonet, Marie-Anne Lelu-Walter, Céline Faugeron, Vincent Gloaguen, Gaëlle Saladin. Physiological responses of the hybrid larch (Larix x eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets. Environmental Science and Pollution Research, 2016, 23 (9), pp.8617-8626. ⟨10.1007/s11356-016-6094-6⟩. ⟨hal-02636839⟩
13 Consultations
65 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More