FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Pasquali, M Beyer, M Logrieco, A Audenaert, K Balmas, V Basler, R Boutigny, AL Chrpova, J Czembor, E Gagkaeva, T Gonzalez-Jaen, M Hofgaard, IS Koycu, ND Hoffmann, L Levic, J Marin, P Miedaner, T Migheli, Q Moretti, A Muller, MEH Munaut, F Parikka, P Pallez-Barthel, M Piec, J Scauflaire, J Scherm, B Stankovic, S Thrane, U Uhlig, S Vanheule, A Yli-Mattila, T Vogelgsang, S AF Pasquali, Matias Beyer, Marco Logrieco, Antonio Audenaert, Kris Balmas, Virgilio Basler, Ryan Boutigny, Anne-Laure Chrpova, Jana Czembor, Elzbieta Gagkaeva, Tatiana Gonzalez-Jaen, Maria Hofgaard, Ingerd S. Koycu, Nagehan D. Hoffmann, Lucien Levic, Jelena Marin, Patricia Miedaner, Thomas Migheli, Quirico Moretti, Antonio Mueller, Marina E. H. Munaut, Francoise Parikka, Paivi Pallez-Barthel, Marine Piec, Jonathan Scauflaire, Jonathan Scherm, Barbara Stankovic, Slavica Thrane, Ulf Uhlig, Silvio Vanheule, Adriaan Yli-Mattila, Tapani Vogelgsang, Susanne TI A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE acetyldeoxynivalenol; chemotype; database; Fusarium; genotype; mycotoxin; nivalenol; trichothecene ID SMALL-GRAIN CEREALS; HEAD BLIGHT PATHOGEN; ALPHA-GENE SEQUENCES; SPECIES COMPLEX; MICRODOCHIUM-NIVALE; FUNGAL PATHOGENS; SOFT WHEAT; PCR ASSAY; CHEMOTYPES; MYCOTOXIN AB Fusarium species, particularly Fusarium graminearum and F culmorum, are the main cause of trichothecene type B contamination in cereals. Data on the distribution of Fusarium trichothecene genotypes in cereals in Europe are scattered in time and space. Furthermore, a common core set of related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis of factors influencing the spatial and temporal population distribution, is lacking. Consequently, based on the available data, it is difficult to identify factors influencing chemotype distribution and spread at the European level. Here we describe the results of a collaborative integrated work which aims (1) to characterize the trichothecene genotypes of strains from three Fusarium species, collected over the period 2000-2013 and (2) to enhance the standardization of epidemiological data collection. Information on host plant, country of origin, sampling location, year of sampling and previous crop of 1147 F graminearurn, 479 F culmorum, and 3 F cortaderiae strains obtained from 17 European countries was compiled and a map of trichothecene type B genotype distribution was plotted for each species. All information on the strains was collected in a freely accessible and updatable database (www.catalogueeu.luxmcc.lu), which will serve as a starting point for epidemiological analysis of potential spatial and temporal trichothecene genotype shifts in Europe. The analysis of the currently available European dataset showed that in F. grarninearum, the predominant genotype was 15-acetyldeoxynivalenol (15-ADON) (82.9%), followed by 3-acetyldeoxynivalenol (3-ADON) (13.6%), and nivalenol (NIV) (3.5%). In F culmorum, the prevalent genotype was 3-ADON (59.9%), while the NIV genotype accounted for the remaining 40.1%. Both, geographical and temporal patterns of trichothecene genotypes distribution were identified. C1 [Pasquali, Matias; Beyer, Marco; Hoffmann, Lucien; Pallez-Barthel, Marine; Piec, Jonathan] Luxembourg Inst Sci & Technol, Dept Environm Res & Innovat, Belvaux, Luxembourg. [Logrieco, Antonio; Moretti, Antonio] CNR, Inst Sci Food Prod, I-70126 Bari, Italy. [Audenaert, Kris; Vanheule, Adriaan] Univ Ghent, Fac Biosci Engn, Dept Appl Biosci, B-9000 Ghent, Belgium. [Balmas, Virgilio; Migheli, Quirico; Scherm, Barbara] Univ Sassari, Dept Agr, I-07100 Sassari, Italy. [Basler, Ryan] INRA, BIOGER UMR, F-78850 Thiverval Grignon, France. [Boutigny, Anne-Laure] ANSES, Plant Hlth Lab, Angers, France. [Chrpova, Jana] Crop Res Inst, Div Crop Genet & Breeding, Prague, Czech Republic. [Czembor, Elzbieta] Nat Res Inst, Plant Breeding & Acclimatizat Inst, Dept Grasses Legumes & Energy Plants, Radzikow, Poland. [Gagkaeva, Tatiana] All Russian Inst Plant Protect, Lab Mycol & Phytopathol, St Petersburg, Russia. [Gonzalez-Jaen, Maria; Marin, Patricia] Univ Complutense Madrid, Fac Biol, Dept Genet, E-28040 Madrid, Spain. [Hofgaard, Ingerd S.] Norwegian Inst Bioecon Res, As, Norway. [Koycu, Nagehan D.] Namik Kemal Univ, Fac Agr, Dept Plant Protect, Tekirdag, Turkey. [Levic, Jelena; Stankovic, Slavica] Maize Res Inst, Lab Phytopathol & Entomol, Belgrade, Serbia. [Miedaner, Thomas] Univ Hohenheim, Plant Breeding Inst, Stuttgart, Germany. [Mueller, Marina E. H.] Inst Landscape Biogeochem, Leibniz Ctr Agr Landscape Res, Muncheberg, Germany. [Munaut, Francoise; Scauflaire, Jonathan] Catholic Univ Louvain, Appl Microbiol Earth & Life Inst, Louvain La Neuve, Belgium. [Parikka, Paivi] Nat Resources Inst Finland Luke, Dept Nat Resources & Bioprod, Jokioinen, Finland. [Thrane, Ulf] Tech Univ Denmark, DTU Syst Biol, Sect Eukaryot Biotechnol, DK-2800 Lyngby, Denmark. [Uhlig, Silvio] Norwegian Vet Inst, Sect Chem & Toxicol, Oslo, Norway. [Yli-Mattila, Tapani] Univ Turku, Dept Biochem, Mol Plant Biol, SF-20500 Turku, Finland. [Vogelgsang, Susanne] Agroscope, Res Div Grassland Sci & Agroecosyst, Inst Sustainabil Sci, Zurich, Switzerland. RP Pasquali, M (reprint author), Luxembourg Inst Sci & Technol, Dept Environm Res & Innovat, Belvaux, Luxembourg.; Vogelgsang, S (reprint author), Agroscope, Res Div Grassland Sci & Agroecosyst, Inst Sustainabil Sci, Zurich, Switzerland. EM matias.pasquali@list.lu; susanne.vogelgsang@agroscope.admin.ch FU Ministere de l'Agriculture, de la Viticulture et de la Protection des Consommateurs-Administration des Services Techniques de l'Agriculture; M.I.U.R. Project AGROGEN (Laboratory of GENomics for traits of AGROnomic importance in durum wheat: Identification of useful genes, functional analysis and assisted selection by biological markers for the development of the national seed chain) [602/Ric]; Felix Thornley Cobbold Trust; John Oldacre Foundation; Ministry of Agriculture of the Czech Republic [800415]; Spanish Ministry MINECO [AGL201.4-53928-C2-2-R]; Ministry of Agriculture and Food, Norway; Federal Ministry of Education and Research (BMBF) (GABI-KANADA), Bonn [FKZ 0313711A]; German Academic Exchange Service (DAAD), Bonn [A/06/92183]; Finnish Ministry of Agriculture and Forestry; Direction Generale de l'Agriculture, Direction de la Recherche [D31-3159, D31-1162, D31-7055]; P.O.R. SARDEGNA F.S.; Danish Directorate for Food, Fisheries and Agri Business [FFS05-3]; Academy of Finland [126917, 131957, 250904, 252162, 267188, 266984]; Olvi Foundation; Turku University Foundation; CIMO travel grant; Nordic network project New Emerging Mycotoxins and Secondary Metabolites in Toxigenic Fungi of Northern Europe - Nordic Research Board [090014] FX The Luxembourg institute of Science and Technology, LU, acknowledges the Ministere de l'Agriculture, de la Viticulture et de la Protection des Consommateurs-Administration des Services Techniques de l'Agriculture for financially supporting the Sentinelle project. The work on Italian strains has been financially supported through the M.I.U.R. Project AGROGEN (Laboratory of GENomics for traits of AGROnomic importance in durum wheat: Identification of useful genes, functional analysis and assisted selection by biological markers for the development of the national seed chain) (D. D. 14.03.2005 n. 602/Ric). Funding for the research of Ryan Basler was provided by Felix Thornley Cobbold Trust and the John Oldacre Foundation.; The work of JC was supported by the Ministry of Agriculture of the Czech Republic, Project No. 800415. The research of MG and PG was supported by the Spanish Ministry MINECO (AGL201.4-53928-C2-2-R). The Ministry of Agriculture and Food, Norway funded the work of IH. The research of TM was funded by the Federal Ministry of Education and Research (BMBF) (GABI-KANADA #FKZ 0313711A), Bonn and by the German Academic Exchange Service (DAAD), Bonn (code no.: A/06/92183). PP acknowledges the Finnish Ministry of Agriculture and Forestry for funding the project FinMyco on Fusarium and mycotoxins in Finland. The research of JS was funded by the Direction Generale de l'Agriculture, Direction de la Recherche (ref. D31-3159, D31-1162, D31-7055), in the framework of a project entitled "Caracterization et dynamique des fusarioses sur mais en Region Wallonne." BS acknowledges support by P.O.R. SARDEGNA F.S.E. 2007-2013-Obiettivo competitivita regionale e occupazione, Asse IV Capitale umano, Linea di Attivita 1.3.1 (research project "Identification of natural and natural-like molecules inhibiting mycotoxin biosynthesis by Fusaria pathogenic on cereals"). UT thanks the Danish Directorate for Food, Fisheries and Agri Business grant FFS05-3 for financial support. The work of TY was financially supported by the Academy of Finland (no. 126917, 131957, 250904, 252162, 267188, and 266984), Olvi Foundation, Turku University Foundation, a CIMO travel grant to Taha Hussien, and the Nordic network project New Emerging Mycotoxins and Secondary Metabolites in Toxigenic Fungi of Northern Europe (project 090014), which was funded by the Nordic Research Board. NR 64 TC 0 Z9 0 U1 0 U2 0 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 6 PY 2016 VL 7 AR 406 DI 10.3389/fmicb.2016.00406 PG 11 WC Microbiology SC Microbiology GA DI4XO UT WOS:000373502900002 ER EF