Skip to Main content Skip to Navigation
Journal articles

Peripheral thyroid hormone levels and hepatic thyroid hormone deiodinase gene expression in dairy heifers on the day of ovulation and during the early peri-implantation period

Abstract : Background: Before the onset of fetal thyroid hormone production, the transplacental delivery of maternal thyroid hormones is necessary for embryonic and fetal development. Therefore, the adaptation of maternal thyroid hormone metabolism may be important for pregnancy success and embryo survival. The aims of this study were to determine the thyroid hormone levels during the early peri-implantation period until day 18 and on the day of ovulation, to determine whether pregnancy success is dependent on a "normothyroid status" and to determine whether physiological adaptations in maternal thyroid hormone metabolism occur, which may be necessary to provide sufficient amounts of biologically active T-3 to support early pregnancy. Therefore, blood samples obtained on the day of ovulation (day 0) and days 14 and 18 of the Holstein-Friesian heifers (n = 10) during the respective pregnant, non-pregnant and negative control cycles were analyzed for thyroid-stimulating-hormone (TSH), thyroxine (T-4) and triiodothyronine (T-3). Liver biopsies (day 18) from pregnant and respective non-pregnant heifers were analyzed for mRNA expression of the most abundant hepatic thyroid hormone deiodinase (DIO1) by real time qPCR. Results: Although liver DIO1 mRNA expression did not differ between the pregnant and non-pregnant heifers on day 18, the serum concentrations of TSH and T-3 on day 18 were higher in non-pregnant heifers compared to pregnant heifers (P < 0.05). Moreover, T-3 decreased between day 0 and 18 in pregnant heifers (P < 0.001). Conclusions: In conclusion, no associations between thyroid hormone patterns on day 18 and pregnancy success were detected. During the early peri-implantation period, TSH and T-3 may be affected by the pregnancy status because both TSH and T-3 were lower on day 18 in pregnant heifers compared to non-pregnant dairy heifers. In further studies, the thyroid hormone axis should be evaluated throughout the entire gestation to confirm these data and identify other possible effects of pregnancy on the thyroid hormone axis in cattle.
Document type :
Journal articles
Complete list of metadata

Cited literature [28 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02637576
Contributor : Migration Prodinra <>
Submitted on : Wednesday, May 27, 2020 - 11:28:18 PM
Last modification on : Tuesday, April 6, 2021 - 1:38:07 PM

File

2016_Meyerholz_Acta Veterinari...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Marie Margarete Meyerholz, Kirsten Mense, Matthias Linden, Mariam Raliou, Olivier Sandra, et al.. Peripheral thyroid hormone levels and hepatic thyroid hormone deiodinase gene expression in dairy heifers on the day of ovulation and during the early peri-implantation period. Acta Veterinaria Scandinavica, BioMed Central, 2016, 58, ⟨10.1186/s13028-016-0231-6⟩. ⟨hal-02637576⟩

Share

Metrics

Record views

26

Files downloads

41