FN Clarivate Analytics Web of Science
VR 1.0
PT J
AU Manousaki, T
   Tsakogiannis, A
   Lagnel, J
   Sarropoulou, E
   Xiang, JZ
   Papandroulakis, N
   Mylonas, CC
   Tsigenopoulos, CS
AF Manousaki, Tereza
   Tsakogiannis, Alexandros
   Lagnel, Jacques
   Sarropoulou, Elena
   Xiang, Jenny Z.
   Papandroulakis, Nikos
   Mylonas, Constantinos C.
   Tsigenopoulos, Costas S.
TI The sex-specific transcriptome of the hermaphrodite sparid sharpsnout
   seabream (Diplodus puntazzo)
SO BMC GENOMICS
LA English
DT Article
DE Sparidae; Sharpsnout seabream; Diplodus puntazzo; RNA-Seq;
   Transcriptome; Gonads; Brain; Sex differentiation; Hermaphroditism
ID PROTANDROUS BLACK PORGY; DIFFERENTIAL EXPRESSION ANALYSIS; SEXUALLY
   DIMORPHIC EXPRESSION; HIGH WATER TEMPERATURE; DM-DOMAIN GENE;
   ACANTHOPAGRUS-SCHLEGELI; RNA-SEQ; MOLECULAR-CLONING; DESERT-HEDGEHOG;
   OVARIAN DEVELOPMENT
AB Background: Teleosts are characterized by a remarkable breadth of sexual mechanisms including various forms of hermaphroditism. Sparidae is a fish family exhibiting gonochorism or hermaphroditism even in closely related species. The sparid Diplodus puntazzo (sharpsnout seabream), exhibits rudimentary hermaphroditism characterized by intersexual immature gonads but single-sex mature ones. Apart from the intriguing reproductive biology, it is economically important with a continuously growing aquaculture in the Mediterranean Sea, but limited available genetic resources. Our aim was to characterize the expressed transcriptome of gonads and brains through RNA-Sequencing and explore the properties of genes that exhibit sex-biased expression profiles.
   Results: Through RNA-Sequencing we obtained an assembled transcriptome of 82,331 loci. The expression analysis uncovered remarkable differences between male and female gonads, while male and female brains were almost identical. Focused search for known targets of sex determination and differentiation in vertebrates built the sex-specific expression profile of sharpsnout seabream. Finally, a thorough genetic marker discovery pipeline led to the retrieval of 85,189 SNPs and 29,076 microsatellites enriching the available genetic markers for this species.
   Conclusions: We obtained a nearly complete source of transcriptomic sequence as well as marker information for sharpsnout seabream, laying the ground for understanding the complex process of sex differentiation of this economically valuable species. The genes involved include known candidates from other vertebrate species, suggesting a conservation of the toolkit between gonochorists and hermaphrodites.
C1 [Manousaki, Tereza; Tsakogiannis, Alexandros; Lagnel, Jacques; Sarropoulou, Elena; Papandroulakis, Nikos; Mylonas, Constantinos C.; Tsigenopoulos, Costas S.] Hellen Ctr Marine Res, Inst Marine Biol Biotechnol & Aquaculture IMBBC, Iraklion, Greece.
   [Xiang, Jenny Z.] Weill Cornell Med Coll, Genom Resources Core Facil, New York, NY USA.
RP Tsigenopoulos, CS (reprint author), Hellen Ctr Marine Res, Inst Marine Biol Biotechnol & Aquaculture IMBBC, Iraklion, Greece.
EM tsigeno@hcmr.gr
OI Mylonas, Constantinos/0000-0003-1805-4392; Papandroulakis,
   Nikos/0000-0002-7109-7132; Tsigenopoulos, Costas/0000-0003-0615-7941
FU Ministry of Education and Religious Affairs [36]; EU; Hellenic Republic
FX Financial support for this study has been provided by the Ministry of
   Education and Religious Affairs, under the Call "ARISTEIA I" of the
   National Strategic Reference Framework 2007-2013 (SPARCOMP, #36),
   co-funded by the EU and the Hellenic Republic through the European
   Social Fund. We would like to thank V. Terzoglou and E. Kaitetzidou for
   help in RNA extractions, Irini Sigelaki for help in sampling and Dr.
   Hooman Moghadam, Dr. Gianpaolo Zampicinini, Dr. Jon B. Kristoffersen and
   Ji Hyoun Kang for valuable discussions. Finally, we would like to thank
   three anonymous reviewers for their valuable comments on the manuscript.
NR 120
TC 22
Z9 22
U1 3
U2 63
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1471-2164
J9 BMC GENOMICS
JI BMC Genomics
PD AUG 6
PY 2014
VL 15
AR 655
DI 10.1186/1471-2164-15-655
PG 16
WC Biotechnology & Applied Microbiology; Genetics & Heredity
SC Biotechnology & Applied Microbiology; Genetics & Heredity
GA AN5PN
UT WOS:000340643900001
PM 25099474
OA gold
DA 2018-01-04
ER

EF