Sustainable bioenergy crops must contribute not only to the production of renewable energy but also to maintaining or restoring water resource and quality. The aim of this study was to quantify water drainage and nitrate leaching under perennial (Miscanthus x giganteus and switchgrass), "semi-perennial" (fescue and alfalfa) and annual (sorghum and triticale) bioenergy crops managed with two N fertilisation rates. Soil water and mineral N contents were measured twice a year during 7 consecutive years. These measurements were used to initialize the STICS model which simulated in turn the amounts of drained water and nitrate leached below 210 cm. Semi-perennial crops produced less drainage than annual crops (64 vs. 133 mm year(-1)) despite a similar biomass production. Perennial crops resulted in an intermediate drainage (90 mm year(-1)) but a greater biomass production. The drainage was negatively correlated with biomass production for perennial and annual crops. Perennial crops exhibited much higher water use efficiency than the other species. Nitrate concentration in drained water was low for all crops, most often less than 20 mg NO3 l(-1). It was lower for perennials than other crops, except for miscanthus on the first year of measurement. However, the comparison of model outputs with nitrate concentrations measured in subsoil after 5 years indicated that the peak of nitrate produced after miscanthus establishment was subsequently recovered by the crop in deep layers (below 210 cm). Perennial bioenergy crops have potential for restoring water quality but may decrease groundwater recharge in deep soils or dry climates.