Characterisation and natural variation of a dehydrin gene in Quercus petraea (Matt.) Liebl. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Plant Biology Année : 2011

Characterisation and natural variation of a dehydrin gene in Quercus petraea (Matt.) Liebl.

Résumé

For the first time in sessile oak [Quercus petraea (Matt.) Liebl.], the isolation and characterisation of a full-length dehydrin gene and its promoter region, as well as its allelic variation in natural populations, is reported. Dehydrins (Dhn) are stress-related genes important for the survival of perennial plants in a seasonal climate. A full-length dehydrin gene (Dhn3) was characterised at the nucleotide level and the protein structure was modelled. Additionally, the allelic variation was analysed in five natural populations of Quercus petraea (Matt.) Liebl. sampled along an altitudinal gradient in the French Pyrenees. The analysed sequences contain typical domains of the K(n) class of dehydrins in the coding region. Also, the 5'untranslated region (promoter) of the gene was amplified, which shows typical motifs essential for drought- and cold-responsive gene expression. Single nucleotide substitutions and indels (insertions/deletions) within the coding region determine large biochemical differences at the protein level. However, only low levels of genetic differentiation between populations from different altitudes were detectable.

Dates et versions

hal-02647308 , version 1 (29-05-2020)

Identifiants

Citer

B. Vornam, O. Gailing, Jérémy Derory, Christophe Plomion, Antoine Kremer, et al.. Characterisation and natural variation of a dehydrin gene in Quercus petraea (Matt.) Liebl.. Plant Biology, 2011, 13 (6), pp.881 - 887. ⟨10.1111/j.1438-8677.2011.00446.x⟩. ⟨hal-02647308⟩

Collections

INRA INRAE BIOGECO
12 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More