Anti-androgen vinclozolin impairs sperm quality and steroidogenesis in goldfish. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Aquatic Toxicology Année : 2012

Anti-androgen vinclozolin impairs sperm quality and steroidogenesis in goldfish.

Résumé

In mammals, vinclozolin (VZ) is known as anti-androgen, which causes male infertility via androgen receptor (AR) antagonism. In aquatic animals, the VZ effects on reproductive functions are largely unknown and results are somewhat contradictory. To understand VZ adverse effects on male reproduction, mature goldfish (Carassius auratus) were exposed to three nominal VZ concentrations (100, 400, and 800 μg/L) and alternations in gonadosomatic (GSI) and hepatosomatic indices (HSI), 17β-estradiol (E(2)), 11-ketotestosterone (11-KT) and sperm quality were investigated compared to the solvent control. One group was exposed to E(2) (nominal concentration of 5 μg/L), an estrogenic compound, as a negative control. Following one month exposure, GSI and HSI were unchanged in all VZ treated groups compared to solvent control. Sperm volume, motility and velocity were reduced in fish exposed to 800 μg/L VZ. This was associated with the decrease in 11-KT level, suggesting direct VZ effects on testicular androgenesis and sperm functions. In goldfish exposed to 100 μg/L VZ, 11-KT was increased but E(2) remained unchanged. This is, probably, the main reason for unchanged sperm quality at 100 μg/L VZ. In goldfish exposed to E(2), GSI and 11-KT were decreased, E(2) was increased and no sperm was produced. The present study shows different dose-dependent VZ effects, which lead to impairment in sperm quality via disruption in steroidogenesis. In addition to VZ effects through competitive binding to AR, our data suggests potential effects of VZ by direct inhibition of 11-KT biosynthesis in fish as well as abnormalities in sperm morphology.

Dates et versions

hal-02647327 , version 1 (29-05-2020)

Identifiants

Citer

Azadeh Hatef, Sayyed Mohammad Hadi Alavi, Sylvain Milla, Jiří Křišťan, Mahdi Golshan, et al.. Anti-androgen vinclozolin impairs sperm quality and steroidogenesis in goldfish.. Aquatic Toxicology, 2012, 122-123, pp.181-187. ⟨10.1016/j.aquatox.2012.06.009⟩. ⟨hal-02647327⟩
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