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MalF is essential for persistence of Mycoplasma gallisepticum in vivo

Abstract : There is limited understanding of the molecular basis of virulence in the important avian pathogen Mycoplasma gallisepticum. To define genes that may be involved in colonization of chickens, a collection of mutants of the virulent Ap3AS strain of M. gallisepticum were generated by signature-tagged transposon mutagenesis. The collection included mutants with single insertions in the genes encoding the adhesin GapA and the cytadherence-related protein CrmA, and Western blotting confirmed that these mutants did not express these proteins. In two separate in vivo screenings, two GapA-deficient mutants (ST mutants 02-1 and 06-1) were occasionally recovered from birds, suggesting that GapA expression may not always be essential for persistence of strain Ap3AS. CrmA-deficient ST mutant 33-1 colonized birds poorly and had reduced virulence, indicating that CrmA was a significant virulence factor, but was not absolutely essential for colonization. ST mutant 04-1 contained a single transposon insertion in malF, a predicted ABC sugar transport permease, and could not be reisolated even when inoculated by itself into a group of birds, suggesting that expression of MalF was essential for persistence of M. galliseptium strain Ap3AS in infected birds.
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Submitted on : Friday, May 29, 2020 - 9:45:13 AM
Last modification on : Tuesday, October 19, 2021 - 2:22:06 PM

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Chi-Wen Tseng, Anna Kanci, Christine Citti, Renate Rosengarten, Chien-Ju Chiu, et al.. MalF is essential for persistence of Mycoplasma gallisepticum in vivo. Microbiology, Microbiology Society, 2013, 159 (Pt 7), pp.1459-70. ⟨10.1099/mic.0.067553-0⟩. ⟨hal-02648509⟩

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