Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Article Dans Une Revue Tropical Animal Health and Production Année : 2011

Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed

Résumé

We evaluated the effect of heat challenge on cell viability, concanavalin A-induced proliferation and heat shock protein (HSPs) mRNA expression in peripheral mononuclear blood cells (PBMC) isolated from Creole (CR) and Large White (LW) pigs. The PBMCs were cultured for 9 h at 37°C before being subjected to heat challenge: (1) at 42°C or 45°C for 2, 4, 6 and 9 h to monitor cell viability;(2) at 45°C for 2 and 9 h followed by stimulation for 24 h at 37°C with concanavalin A to evaluate mitogen-induced proliferation; and (3) at 45°C for 3, 6 and 9 h to measure induction of HSP70.2 and HSP90 mRNA. Cell viability was affected by breed and temperature (P < 0.01), and the viability decrease caused by heat challenge was greater for LW than CR pigs. For mitogen-stimulated PBMCs, incubation at 45°C reduced lymphoblastogenesis equally in both breeds (P < 0.01). Although heat challenge for 3 and 6 h at 45°C induced expression of HSP70.2 and HSP90 mRNA, no breed difference was observed. In conclusion, differences in heat resistance between these two breeds at the whole organism level are reflected at the cellular level. Neither HSP70.2 nor HSP90 mRNA expression levels explain this effect.

Dates et versions

hal-02649034 , version 1 (29-05-2020)

Identifiants

Citer

Jean-Christophe Bambou, Jean-Luc Gourdine, Roxanne Grondin, Nathalie Vachiery, David Renaudeau. Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed. Tropical Animal Health and Production, 2011, 43 (8), pp.1535-1541. ⟨10.1007/s11250-011-9838-9⟩. ⟨hal-02649034⟩
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