Viral sanitary status of declining grapevine Syrah clones and genetic diversity of Grapevine Rupestris stem pitting-associated virus - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue European Journal of Plant Pathology Année : 2013

Viral sanitary status of declining grapevine Syrah clones and genetic diversity of Grapevine Rupestris stem pitting-associated virus

Statut sanitaire virale de la baisse des clones vigne de Syrah et de la diversité génétique des souches de vigne Rupestris virus piqûres associée

Résumé

In order to determine the etiology of Syrah decline, virus detection was performed on 22 Syrah clones, chosen for their various levels of sensitivity to Syrah decline. All clones, including the sensitive ones, were free of 20 main grapevine viruses. In contrast, Grapevine Syrah virus-1 and Grapevine Rupestris stem pitting-associated virus (GRSPaV), were detected in 56 % and 100 % of the analysed Syrah clones respectively. This is the first report of GSyV-1 in a French vineyard. The genetic diversity of a 380 nt region within the GRSPaV coat protein gene was studied extensively in vines differing in their sensitivity to Syrah decline. Most GRSPaV variants were scattered between the four phylogenetic groups previously identified; 65 % of the sequences analysed were found to belong to the GRSPaV-group 1, 22 % to-group 2b, 10 % to-group 2a and 2 % to-group 3. Seventy percent of the 31 plants analysed harboured mixtures of genomic variants. Statistical analyses revealed no significant correlation between sensitivity and GRSPaV sequence variation. This suggests that GRSPaV is not the direct etiological agent of the Syrah decline.

Dates et versions

hal-02649580 , version 1 (29-05-2020)

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Monique M. Beuve, Benoît Moury, Anne-Sophie Spilmont, Lauriane Sempé-Ignatovic, Caroline Hemmer, et al.. Viral sanitary status of declining grapevine Syrah clones and genetic diversity of Grapevine Rupestris stem pitting-associated virus. European Journal of Plant Pathology, 2013, 135, pp.439-452. ⟨10.1007/s10658-012-0101-7⟩. ⟨hal-02649580⟩
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