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RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

Olle Terenius 1 Alexie Papanicolaou 2, 3 Jennie S. Garbutt 4 Ioannis Eleftherianos 5 Hanneke Huvenne 6 Sriramana Kanginakudru 7 Merete Albrechtsen 8 Chunju An 9 Jean-Luc Aymeric 10 Andrea Barthel 11 Piotr Bebas 12 Kavita Bitra 11 Alejandra Bravo 13 Francois Chevalieri 14 Derek P. Collinge 3, 15 Cristina M. Crava 16 Ruud A. de Maagd 17 Bernard Duvic 10 Martin Erlandson 18, 19 Ingrid Faye 20 Gabriella Felfoeldi 21 Haruhiko Fujiwara 22 Ryo Futahashi 23, 24 Archana S. Gandhe 7 Heather S. Gatehouse 25 Laurence N. Gatehouse 25 Jadwiga M. Giebultowicz 26 Isabel Gómez 13 Cornelis J. P. Grimmelikhuijzen 27 Astrid T. Groot 11 Frank Hauser 27 David G. Heckel 11 Dwayne D. Hegedus 18 Steven Hrycaj Lihua Huang J. Joe Hull Kostas Iatrou Masatoshi Iga 6 Michael R. Kanost 9 Joanna Kotwica 12 Changyou Li Jianghong Li Jisheng Liu 6 Magnus Lundmark 8 Shogo Matsumoto Martina Meyering-Vos Peter J. Millichap 4 Antonia Monteiro Nirotpal Mrinal 7 Teruyuki Niimi Daniela Nowara 27 Atsushi Ohnishi Vicencio Oostra Katsuhisa Ozaki Maria Papakonstantinou Aleksandar Popadic Manchikatla V. Rajam Suzanne Saenko Robert M. Simpson 25 Mario Soberon 13 Michael R. Strand 11 Shuichiro Tomita Umut Toprak 19 Ping Wang Choon Wei Wee Steven Whyard Wenqing Zhang Javaregowda Nagaraju 7 Richard H. Ffrench-Constant 2 Salvador Herrero 16, 17 Karl Gordon 3 Luc Swelters Guy Smagghe 6
Abstract : Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. (C) 2010 Elsevier Ltd. All rights reserved.
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Olle Terenius, Alexie Papanicolaou, Jennie S. Garbutt, Ioannis Eleftherianos, Hanneke Huvenne, et al.. RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design. Journal of Insect Physiology, Elsevier, 2011, 57 (2), pp.231 - 245. ⟨10.1016/j.jinsphys.2010.11.006⟩. ⟨hal-02652706⟩

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