Prion protein in the cerebrospinal fluid of healthy and naturally scrapie-affected sheep - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Journal of General Virology Année : 2006

Prion protein in the cerebrospinal fluid of healthy and naturally scrapie-affected sheep

Résumé

The aim of this study was to characterize the cerebrospinal fluid (CSF) prion protein (PrP) of healthy and naturally scrapie-affected sheep. The soluble form of CSF PrPC immunoblotted with an anti-octarepeat and an anti-C terminus mAb showed two isoforms of approximately 33 and 26 kDa, corresponding to the biglycosylated and unglycosylated isoforms of brain PrPC. Neither the mean concentration nor the electrophoretic profile of CSF PrP differed between healthy and scrapieaffected sheep, whereas a slightly increased resistance of CSF PrP to mild proteolysis by proteinase K was evident in the CSF of scrapie-affected sheep. No difference in susceptibility to proteolysis was observed between the two ARR and VRQ genetic variants of the purified prokaryote recombinant PrP. It was concluded that the physicochemical properties of PrPC in the CSF could be altered during scrapie and that these changes might reflect the physiopathological process of prion disease.

Domaines

Virologie

Dates et versions

hal-02655586 , version 1 (29-05-2020)

Identifiants

Citer

Nicole Picard-Hagen, Véronique V. Gayrard-Troy, Catherine Viguié, Mohammed M. Moudjou, Chantal Imbs, et al.. Prion protein in the cerebrospinal fluid of healthy and naturally scrapie-affected sheep. Journal of General Virology, 2006, 87 (12), pp.3723-3727. ⟨10.1099/vir.0.81859-0⟩. ⟨hal-02655586⟩
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